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| | ===Results=== | | ===Results=== |
| | ''Maria and Afton'' | | ''Maria and Afton'' |
| - | *Digestion was successful on parts J23106 and J06702 | + | * Digestion was successful on parts J23106 and J06702 |
| | + | * Gel Electrophoresis Results |
| | + | [[Image:2009-07-13 digest05 106and702.jpg|350px|thumb|none|from left to right: 100bp Ladder,J23106,J06702,J06702]] |
| | | | |
| | [[User:Trentay|Trentay]] 18:30, 13 July 2009 (UTC) | | [[User:Trentay|Trentay]] 18:30, 13 July 2009 (UTC) |
| | + | |
| | + | ''Craig and Clay'' |
| | + | * The parts that were plated on Friday were unsuccessful. Electrotransformation will be performed again. |
| | ---- | | ---- |
| | | | |
| Line 55: |
Line 60: |
| | ''Kevin and Adam'' | | ''Kevin and Adam'' |
| | * Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock. | | * Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock. |
| - | * 2 | + | |
| - | ---- | + | |
| | + | ''Craig and Clay'' |
| | + | * A second attempt at electrotransformation of parts BBa_I742111, BBa_K118024, and BBa_K118025 will be performed today. These electrotransformed parts will be plated on ampicillin plates. |
| | + | * If the above is unsuccessful, the parts will be re-plated on the ampicillin plates and on non-selective plates. |
| | + | |
| | + | ---- |
| | | | |
| | ====Wetlab==== | | ====Wetlab==== |
| Line 64: |
Line 74: |
| | * Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) | | * Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) |
| | * Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone) | | * Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone) |
| - | * Plated: | + | * Plated, Controls and NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5) |
| - | ** (+) Control: NEB 10 beta on LB
| + | |
| - | ** (+) Control: NEB 10 beta on LB shocked
| + | {| cellpadding="5" border="1" padding="5" |
| - | ** (-) Control: NEB 10 beta on Cm
| + | |+ |
| - | ** NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
| + | |- |
| | + | ! Name !! Plate !! Growth Observation |
| | + | |- |
| | + | | Control (+) NEB10β || LB || n/a |
| | + | |- |
| | + | | Control (+) NEB10β shocked || LB || n/a |
| | + | |- |
| | + | | Control (-) NEB10β || Cm || n/a |
| | + | |- |
| | + | | J23106/J06702/pSB4C5 ligation || Cm || n/a |
| | + | |} |
| | [[User:Trentay|Trentay]] 20:26, 13 July 2009 (UTC) | | [[User:Trentay|Trentay]] 20:26, 13 July 2009 (UTC) |
| | | | |
| Line 74: |
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| | *grew plasmids pSB4C5, E0240, and J06702 from glycerol stocks. Will be mini prepped in the morning. | | *grew plasmids pSB4C5, E0240, and J06702 from glycerol stocks. Will be mini prepped in the morning. |
| | [[User:Bussingkm|Bussingkm]] 22:59, 13 July 2009 (UTC) | | [[User:Bussingkm|Bussingkm]] 22:59, 13 July 2009 (UTC) |
| | + | |
| | + | ''Craig and Clay'' |
| | + | * The three parts related to limonene synthesis were electrotransformed again and plated on ampicillin plates. |
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| [http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15]
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| [http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26]
| [http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]
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| [http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2]
| [http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3]
| [http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4]
| [http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5]
| [http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
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| [http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9]
| [http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10]
| [http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11]
| [http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12]
| [http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
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| [http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16]
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| [http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18]
| [http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19]
| [http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
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| [http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25]
| [http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26]
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| [http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
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| [http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]
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| July
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2]
| [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
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| [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7]
| [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9]
| [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
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| [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14]
| [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16]
| [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
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| [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23]
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| [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30]
| [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]
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| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
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| [http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4]
| [http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5]
| [http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8]
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| [http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10]
| [http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11]
| [http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12]
| [http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
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| [http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18]
| [http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19]
| [http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22]
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| [http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25]
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| [http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27]
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| [http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2]
| [http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3]
| [http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5]
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| [http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9]
| [http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10]
| [http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11]
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| [http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16]
| [http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17]
| [http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18]
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| [http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22]
| [http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24]
| [http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25]
| [http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
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| [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
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| [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7]
| [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9]
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| [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14]
| [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16]
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| [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
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| [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22]
| [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24]
| [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
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| [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]
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Monday 13 July 2009
Results
Maria and Afton
- Digestion was successful on parts J23106 and J06702
- Gel Electrophoresis Results
from left to right: 100bp Ladder,J23106,J06702,J06702
Trentay 18:30, 13 July 2009 (UTC)
Craig and Clay
- The parts that were plated on Friday were unsuccessful. Electrotransformation will be performed again.
Tasks
Maria and Afton
- Make daily planner for experimentation
- Perform Electrophoresis test on Digested parts J23106 and J06702
- Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Transform ligated DNA into e. coli NEB 10 beta
Trentay 18:34, 13 July 2009 (UTC)
Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock.
Craig and Clay
- A second attempt at electrotransformation of parts BBa_I742111, BBa_K118024, and BBa_K118025 will be performed today. These electrotransformed parts will be plated on ampicillin plates.
- If the above is unsuccessful, the parts will be re-plated on the ampicillin plates and on non-selective plates.
Wetlab
Maria and Afton
- Gel Electrophoresis
- Digestion of J23100
- Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone)
- Plated, Controls and NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
| Name | Plate | Growth Observation
|
| Control (+) NEB10β | LB | n/a
|
| Control (+) NEB10β shocked | LB | n/a
|
| Control (-) NEB10β | Cm | n/a
|
| J23106/J06702/pSB4C5 ligation | Cm | n/a
|
Trentay 20:26, 13 July 2009 (UTC)
Kevin and Adam
- grew plasmids pSB4C5, E0240, and J06702 from glycerol stocks. Will be mini prepped in the morning.
Bussingkm 22:59, 13 July 2009 (UTC)
Craig and Clay
- The three parts related to limonene synthesis were electrotransformed again and plated on ampicillin plates.
|
"
