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Virginia Commonwealth/17 July 2009
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(New page: ==Thursday 16 July 2009== ===Results=== * Results of overnight culture was successful ---- ===Tasks=== ''Maria and Afton'' * Religate promoters that did not grow in the first transforma...) |
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| + | ==Friday 17 July 2009== | ||
===Results=== | ===Results=== | ||
* Results of overnight culture was successful | * Results of overnight culture was successful | ||
| - | + | * NEB 10 beta electrocompetency was successful | |
| + | ** NEB on LB had lawn growth | ||
| + | ** NEB on LB after being shocked had lawn growth | ||
| + | * Electrophoresis test on J06702 and pSB4C5 | ||
| + | ** A DNA band was visible for both | ||
| + | [[User:Trentay|Trentay]] 16:54, 17 July 2009 (UTC) | ||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * There is still no growth on the kanamycin and chloramphenicol plates. | ||
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * Religate promoters that did not grow in the first transformation | + | * Religate promoters that did not grow in the first transformation (backbone pSB4C5) |
| - | * Run an electrophoresis test on | + | ** J23101 w/ E0240 |
| + | ** J23105 w/ E0240 | ||
| + | * Run an electrophoresis test on J06702 and pSB4C5 | ||
| + | [[User:Trentay|Trentay]] 15:53, 17 July 2009 (UTC) | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * Cryogenic stock of the following BioBricks was made | + | * Cryogenic stock of the following BioBricks was made with backbone pSB4C5 (2 vials each) |
| - | + | ||
| - | + | {| cellpadding="5" border="1" padding="5" | |
| - | + | |+ | |
| - | + | |- | |
| - | + | ! Promoter !! RET | |
| - | + | |- | |
| - | + | | J23101 || J06702 | |
| - | + | |- | |
| - | + | | J23102 || E0240 | |
| - | + | |- | |
| + | | J23102 || J06702 | ||
| + | |- | ||
| + | | J23103 || E0240 | ||
| + | |- | ||
| + | | J23103 || J06702 | ||
| + | |- | ||
| + | | J23104 || E0240 | ||
| + | |- | ||
| + | | J23104 || J06702 | ||
| + | |- | ||
| + | | J23105 || J06702 | ||
| + | |- | ||
| + | | J23107 || E0240 | ||
| + | |- | ||
| + | | J23107 || J06702 | ||
| + | |} | ||
| + | * Electrophoresis test | ||
| + | [[User:Trentay|Trentay]] 15:53, 17 July 2009 (UTC) | ||
Latest revision as of 16:50, 10 August 2009
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Contents |
Friday 17 July 2009
Results
- Results of overnight culture was successful
- NEB 10 beta electrocompetency was successful
- NEB on LB had lawn growth
- NEB on LB after being shocked had lawn growth
- Electrophoresis test on J06702 and pSB4C5
- A DNA band was visible for both
Trentay 16:54, 17 July 2009 (UTC)
Craig and Clay
- There is still no growth on the kanamycin and chloramphenicol plates.
Tasks
Maria and Afton
- Religate promoters that did not grow in the first transformation (backbone pSB4C5)
- J23101 w/ E0240
- J23105 w/ E0240
- Run an electrophoresis test on J06702 and pSB4C5
Trentay 15:53, 17 July 2009 (UTC)
Wetlab
Maria and Afton
- Cryogenic stock of the following BioBricks was made with backbone pSB4C5 (2 vials each)
| Promoter | RET |
|---|---|
| J23101 | J06702 |
| J23102 | E0240 |
| J23102 | J06702 |
| J23103 | E0240 |
| J23103 | J06702 |
| J23104 | E0240 |
| J23104 | J06702 |
| J23105 | J06702 |
| J23107 | E0240 |
| J23107 | J06702 |
- Electrophoresis test
Trentay 15:53, 17 July 2009 (UTC)
