Team:Warsaw/Calendar-Main/14 July 2009

From 2009.igem.org

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<!-- TU PISZ CO CHCESZ! -->
<!-- TU PISZ CO CHCESZ! -->
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===Cloning p53 coding sequence===
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<html><h3><div style="text-align: center;">Making of the RBS-cI</div></h3>
-
 
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<h4>Jarek</h4>
-
'''Marcin'''
+
<br />
-
 
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<p>Tasks:</p>
-
 
+
<ul>
-
Task 1:
+
<li>Separation of digested DNA in 0,8% agarose gel.</li></ul>
-
*Transformation of chemocompetent strain of E. coli by plasmid containing p53 sequence
+
-
 
+
-
Procedure:
+
-
 
+
-
*The protocol of transformation has not been changed, except of giving 10 &micro;l of ligated plasmid solution to the bacteria. If you want to see detailed procedure go [https://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 here]
+
-
*Bacteria was plated on the medium containing ampicillin, X-Gal and IPTG
+
-
 
+
-
Task 2: Restriction digest of pKS vector for future aplications:
 
-
Methods:
 
-
* Reaction mixture composition: 2 &mu;l pKS plasmid, 1 &mu;l XbaI (Fermentas), 1 &mu;l SmaI (Fermentas) 2 &mu;l Buffer Tango (Fermentas), 14 &mu;l MQ water
 
-
 
-
Program:
 
 +
<html>
 +
<h3>Cloning of p53 coding sequence</h3>
 +
<h4>Marcin</h4>
 +
<p>Task 1:</p>
 +
<br/>
 +
<ul>
 +
<li>Transformation of chemocompetent strain of E. coli by plasmid containing p53 sequence</li>
 +
</ul>
 +
<br/>
 +
<p>Methods</p>
 +
<ul>
 +
<li>The protocol of transformation has not been changed, except of giving 10 &micro;l of ligated plasmid solution to the bacteria. If you want to see detailed procedure go <a href="https://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009">here</a><li>
 +
<li>Bacteria was plated on the medium containing ampicillin, X-Gal and IPTG</li>
 +
</ul>
 +
<br/>
 +
<p>Task 2: Restriction digest of pKS vector for future aplications:</p>
 +
<br/>
 +
<p>Methods:</p>
 +
<ul>
 +
<li>Reaction mixture composition:
 +
<pre>2 &mu;l pKS plasmid
 +
1 &mu;l XbaI (Fermentas)
 +
1 &mu;l SmaI (Fermentas)
 +
2 &mu;l Buffer Tango (Fermentas)
 +
14 &mu;l MQ water</pre></ul>
 +
<br/>
 +
<p>Program:</p>
 +
<br/>
digest:
digest:
<pre>
<pre>
Line 55: Line 71:
2. 65&deg;C - 15 minutes
2. 65&deg;C - 15 minutes
</pre>
</pre>
 +
<br/>
 +
<h3>Assembly of endosomal detection operon</h3>
 +
<h4>Marcin</h4>
 +
<br/>
 +
<p>Task 1:</p>
 +
<ul>
 +
<li>Breed bacteria to isolate plasmid containing biobricks essential for build the operon:</li></ul>
 +
<p> <a href="http://partsregistry.org/Part:BBa_B0032"><span style="color: black">BBa_B0032</a></span></p>
 +
<p> <a href="http://partsregistry.org/Part:BBa_C0040"><span style="color: black">BBa_c0040</a></span></p>
 +
<p> <a href="http://partsregistry.org/Part:BBa_C0051"><span style="color: black">BBa_C0051</a></span></p>
 +
<p> <a href="http://partsregistry.org/Part:BBa_E0032"><span style="color: black">BBa_E0032</a></span></p>
-
===Assembly of endosomal detection operon===
+
<p>Methods:</p>
 +
<ol>
 +
<li>Prepare LB medium with ampicillin</li>
 +
<li>Add 3.5 ml of the medium to the probes</li>
 +
<li>Add one bacterial colony to each probe</li>
 +
<li>Breed the bacteria about 10 hours</li>
 +
</ol>
-
'''Marcin'''
+
</html>
-
 
+
-
Task 1:
+
-
*Breed bacteria to isolate plasmid containing biobricks essential for build the operon:
+
-
[http://partsregistry.org/Part:BBa_B0032 <span style="color: black;">BBa_B0032</span>]
+
-
 
+
-
[http://partsregistry.org/Part:BBa_C0040 <span style="color: black;">BBa_C0040</span>]
+
-
 
+
-
[http://partsregistry.org/Part:BBa_C0051 <span style="color: black;">BBa_C0051</span>]
+
-
 
+
-
[http://partsregistry.org/Part:BBa_E0032 <span style="color: black;">BBa_E0032</span>]
+
-
 
+
-
Methods:
+
-
# Prepare LB medium with ampicillin
+
-
# Add 3.5 ml of the medium to the probes
+
-
# Add one bacterial colony to each probe
+
-
# Breed the bacteria about 10 hours
+
<html>
<html>
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<ol>
<ol>
<li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
<li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
-
<li>ligation</li>
+
<li>ligation</li></ol>
<p>NOTES: The roughly 1,5kb fragment (b) represents the digested plasmid, the bearly visible fragment (c) weighing about 0,5kb is the  
<p>NOTES: The roughly 1,5kb fragment (b) represents the digested plasmid, the bearly visible fragment (c) weighing about 0,5kb is the  
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-
 
+
</ul>
 +
</li>
 +
</ul>
 +
</li>
</html>
</html>
 +
<h3> <div style="text-align: center;">Construction of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 K177012] operon1_part2 </div></h3>
 +
 +
<h4>Ania</h4>
 +
 +
Tasks:
 +
 +
* Set up a liquid culture from traqnsformants containing [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] on the pSB1A2 ampicillin resistant plasmid. 
 +
 +
* Alkaline lysis of bacterial cultures to obtain plasmids containing following bricks:
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] on the pSB1A2 ampicillin resistant plasmid. 
 +
 +
* Digest of [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] on the pSB1A2 ampicillin resistant plasmid (XbaI/PstI = prospective insert)
 +
* Digest of [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032 - RBS.3] on the pSB1A2 ampicillin resistant plasmid (SpeI/PstI = prospective  vector)
 +
 +
Results:
 +
 +
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032 - RBS.3] properly digested.
 +
 +
 +
===<div style="text-align: center;"><h3>Isolation of BioBricks from 2008 and 2009 Kit Plates</h3></div>===
 +
 +
'''<h4>Monika</h4>'''
 +
 +
 +
Results from 13 July 2009
 +
*Isolation of GFP coding device switched on by IPTG - [http://partsregistry.org/Part:BBa_I763004 <span style="color: blue;">BBa_I763004</span>] was unsuccessful
 +
 +
 +
Tasks:
 +
*Alkaline lysis of bacterial cultures to obtain promoter lambda (cI regulated) with RFP reporter [http://partsregistry.org/Part:BBa_I763007 <span style="color: blue;"> BBa_I763007</span>] (procedure described [https://2009.igem.org/Wiki/Team:Warsaw/protocols#Alkaline_lysis <span style="color: blue;">here</span>])
 +
*Control digestion of  [http://partsregistry.org/Part:BBa_I763007 <span style="color: blue;"> BBa_I763007</span>],  [http://partsregistry.org/Part:BBa_E0840 <span style="color: blue;">  BBa_E0840</span>],  [http://partsregistry.org/Part:BBa_J07037 <span style="color: blue;">  BBa_J07037</span>] and [http://partsregistry.org/Part:BBa_B0024 <span style="color: blue;">BBa_B0024</span>]
 +
 +
 +
Methods
 +
*Digestion of plsmids containing BioBricks by following enzymes (Fermentas) in proper buffers for 2h in 37&deg;C
 +
{| class="wikitable" style="text-align:center; width:500px; height:90px; border-collapse: collapse; border: 1px dashed blue; margin: 1em auto 1em auto" border="1"
 +
|-
 +
!  DNA sample
 +
!  restriction enzymes
 +
!  expected fragments [bp]
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_I763007 <span style="color: blue;"> BBa_I763007</span>]
 +
|  NcoI,  XbaI
 +
|  500,  2497
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_I763007 <span style="color: blue;"> BBa_I763007</span>]
 +
|  EcoRI,  PstI
 +
|  959,  2038
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_E0840 <span style="color: blue;"> BBa_E0840</span>]
 +
|  HincII,  XbaI
 +
|  516, 2441
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_E0840 <span style="color: blue;"> BBa_E0840</span>]
 +
|  EcoRI,  PstI
 +
|  919,  2038
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_J07037 <span style="color: blue;"> BBa_J07037</span>]
 +
|  NcoI,  PstI
 +
|  711,  4626
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_J07037 <span style="color: blue;"> BBa_J07037</span>]
 +
|  EcoRI,  PstI
 +
|  953,  4384
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_B0024  <span style="color: blue;"> BBa_B0024 </span>]
 +
|  EcoRI,  PstI
 +
|  136,  2038
 +
|}
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 21:38, 19 September 2009


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Contents

Creating devices to test promoters in E. coli strains (devices [http://partsregistry.org/Part:BBa_K177024 BBa_K177024] and [http://partsregistry.org/Part:BBa_K177025 BBa_K177025])

Franek


Tasks:

  • Transformation of competent cells with [http://partsregistry.org/Part:BBa_K177024 BBa_K177024] and [http://partsregistry.org/Part:BBa_K177025 BBa_K177025]


Methods:

  • Chemocompetent E. coli cells from Top10 strain were transformed according to our standard procedure with either [http://partsregistry.org/Part:BBa_K177024 BBa_K177024] or [http://partsregistry.org/Part:BBa_K177025 BBa_K177025]
  • Tranformants with [http://partsregistry.org/Part:BBa_K177024 BBa_K177024] were plated on 18 plates with LB, agar-agar and IPTG
  • Tranformants with [http://partsregistry.org/Part:BBa_K177025 BBa_K177025] were plated on 18 plates with LB, agar-agar and 0.2 % arabinose.


Results:

  • Positive selection will be made according to fluorescence under UV light

Making of the RBS-cI

Jarek


Tasks:

  • Separation of digested DNA in 0,8% agarose gel.

Cloning of p53 coding sequence

Marcin

Task 1:


  • Transformation of chemocompetent strain of E. coli by plasmid containing p53 sequence

Methods

  • The protocol of transformation has not been changed, except of giving 10 µl of ligated plasmid solution to the bacteria. If you want to see detailed procedure go here
  • Bacteria was plated on the medium containing ampicillin, X-Gal and IPTG

Task 2: Restriction digest of pKS vector for future aplications:


Methods:

  • Reaction mixture composition: 
    2 μl pKS plasmid
1 μl XbaI (Fermentas)
1 μl SmaI (Fermentas) 
2 μl Buffer Tango (Fermentas)
14 μl MQ water

Program:


digest: 
1. 37°C - 8 hours
2. 65°C - 15 minutes

Assembly of endosomal detection operon

Marcin


Task 1:

  • Breed bacteria to isolate plasmid containing biobricks essential for build the operon:

BBa_B0032

BBa_c0040

BBa_C0051

BBa_E0032

Methods:

  1. Prepare LB medium with ampicillin
  2. Add 3.5 ml of the medium to the probes
  3. Add one bacterial colony to each probe
  4. Breed the bacteria about 10 hours

Cloning of the mgtc promoter into the pKSII+ plasmid

Kamil


Tasks:

  • Ligation verification
  • Bacteria transformation

Methods:

  • Correctness of the ligation was verified with gel electrophoresis on 1% agarose gel.

  • A 200μl batch of chemocompetent bacteria was transformed with 5μl of ligation mix and incubated overnight on petri dishes containing LB medium supplemented with ampicilin, X-gal and IPTG.


Results:

Electrophoresis results:

From left:

  1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
  2. ligation

NOTES: The roughly 1,5kb fragment (b) represents the digested plasmid, the bearly visible fragment (c) weighing about 0,5kb is the digaested mgtc promoter and the (a) band represents the anticipated closed plasmid (dragged back on the electrophoresis due to it's circular natuire).


Conclusions:

  • The new ligation method has proven to be successful and the sample is good to be used for transformation.

Construction of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 K177012] operon1_part2

Ania

Tasks:

  • Set up a liquid culture from traqnsformants containing [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] on the pSB1A2 ampicillin resistant plasmid.
  • Alkaline lysis of bacterial cultures to obtain plasmids containing following bricks:

[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] on the pSB1A2 ampicillin resistant plasmid.

  • Digest of [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] on the pSB1A2 ampicillin resistant plasmid (XbaI/PstI = prospective insert)
  • Digest of [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032 - RBS.3] on the pSB1A2 ampicillin resistant plasmid (SpeI/PstI = prospective vector)

Results:

  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012 - lacI repressor] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032 - RBS.3] properly digested.


Isolation of BioBricks from 2008 and 2009 Kit Plates

Monika


Results from 13 July 2009

  • Isolation of GFP coding device switched on by IPTG - [http://partsregistry.org/Part:BBa_I763004 BBa_I763004] was unsuccessful


Tasks:

  • Alkaline lysis of bacterial cultures to obtain promoter lambda (cI regulated) with RFP reporter [http://partsregistry.org/Part:BBa_I763007 BBa_I763007] (procedure described here)
  • Control digestion of [http://partsregistry.org/Part:BBa_I763007 BBa_I763007], [http://partsregistry.org/Part:BBa_E0840 BBa_E0840], [http://partsregistry.org/Part:BBa_J07037 BBa_J07037] and [http://partsregistry.org/Part:BBa_B0024 BBa_B0024]


Methods

  • Digestion of plsmids containing BioBricks by following enzymes (Fermentas) in proper buffers for 2h in 37°C
DNA sample restriction enzymes expected fragments [bp]
[http://partsregistry.org/Part:BBa_I763007 BBa_I763007] NcoI, XbaI 500, 2497
[http://partsregistry.org/Part:BBa_I763007 BBa_I763007] EcoRI, PstI 959, 2038
[http://partsregistry.org/Part:BBa_E0840 BBa_E0840] HincII, XbaI 516, 2441
[http://partsregistry.org/Part:BBa_E0840 BBa_E0840] EcoRI, PstI 919, 2038
[http://partsregistry.org/Part:BBa_J07037 BBa_J07037] NcoI, PstI 711, 4626
[http://partsregistry.org/Part:BBa_J07037 BBa_J07037] EcoRI, PstI 953, 4384
[http://partsregistry.org/Part:BBa_B0024 BBa_B0024 ] EcoRI, PstI 136, 2038


April
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_April_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_April_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_April_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_April_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_April_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_April_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_April_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_April_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_April_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_April_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_April_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_April_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_April_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_April_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_April_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_April_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_April_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_April_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_April_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_April_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_April_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_April_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_April_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_April_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_April_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_April_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_April_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_April_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_April_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_April_2009 30]
May
MTWTFSS
        [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_May_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_May_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_May_2009 3]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/4_May_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_May_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_May_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_May_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_May_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_May_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_May_2009 10]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/11_May_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_May_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_May_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_May_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_May_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_May_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_May_2009 17]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/18_May_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_May_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_May_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_May_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_May_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_May_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_May_2009 24]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/25_May_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_May_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_May_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_May_2009 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_June_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_June_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_June_2009 28]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/29_June_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_July_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_July_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_July_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_July_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_July_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_July_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_July_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_July_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_July_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_July_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_July_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_July_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_July_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_July_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_July_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_July_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_July_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_July_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_July_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_July_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_July_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_July_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_July_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_July_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_July_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_July_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_July_2009 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_July_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_August_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_August_2009 2]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/3_August_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_August_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_August_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_August_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_August_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_August_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_August_2009 9]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/10_August_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_August_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_August_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_August_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_August_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_August_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_August_2009 16]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/17_August_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_August_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_August_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_August_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_August_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_August_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_August_2009 23]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/24_August_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_August_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_August_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_August_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_August_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_August_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_August_2009 30]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_September_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_September_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_September_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_September_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_September_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_September_2009 6]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/7_September_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_September_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_September_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_September_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_September_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_September_2009 13]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/14_September_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_September_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_September_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_September_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_September_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_September_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_September_2009 20]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/21_September_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_September_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_September_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_September_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_September_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_September_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_September_2009 27]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/28_September_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_September_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_October_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_October_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_October_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_October_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_October_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/28_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
Retrieved from "http://2009.igem.org/Team:Warsaw/Calendar-Main/14_July_2009"