Team:Calgary/26 July 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 22:55, 20 October 2009

University of Calgary

THIS MONTH

July
M	T	W	T	F	S	S
		[http://2009.igem.org/Team:Calgary/1_July_2009 1]	[http://2009.igem.org/Team:Calgary/2_July_2009 2]	[http://2009.igem.org/Team:Calgary/3_July_2009 3]	[http://2009.igem.org/Team:Calgary/4_July_2009 4]	[http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6]	[http://2009.igem.org/Team:Calgary/7_July_2009 7]	[http://2009.igem.org/Team:Calgary/8_July_2009 8]	[http://2009.igem.org/Team:Calgary/9_July_2009 9]	[http://2009.igem.org/Team:Calgary/10_July_2009 10]	[http://2009.igem.org/Team:Calgary/11_July_2009 11]	[http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13]	[http://2009.igem.org/Team:Calgary/14_July_2009 14]	[http://2009.igem.org/Team:Calgary/15_July_2009 15]	[http://2009.igem.org/Team:Calgary/16_July_2009 16]	[http://2009.igem.org/Team:Calgary/17_July_2009 17]	[http://2009.igem.org/Team:Calgary/18_July_2009 18]	[http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20]	[http://2009.igem.org/Team:Calgary/21_July_2009 21]	[http://2009.igem.org/Team:Calgary/22_July_2009 22]	[http://2009.igem.org/Team:Calgary/23_July_2009 23]	[http://2009.igem.org/Team:Calgary/24_July_2009 24]	[http://2009.igem.org/Team:Calgary/25_July_2009 25]	[http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27]	[http://2009.igem.org/Team:Calgary/28_July_2009 28]	[http://2009.igem.org/Team:Calgary/29_July_2009 29]	[http://2009.igem.org/Team:Calgary/30_July_2009 30]	[http://2009.igem.org/Team:Calgary/31_July_2009 31]

NOTEBOOK PAGE INDEX

Carol
Chinmoyee
Emily
Fahd
Iman
Jamie
Jeremy
Katie
Kevin
Mandy
Patrick
Prima
Stefan
Vicki

CALENDAR

May
M	T	W	T	F	S	S
				[http://2009.igem.org/Team:Calgary/1_May_2009 1]	[http://2009.igem.org/Team:Calgary/2_May_2009 2]	[http://2009.igem.org/Team:Calgary/3_May_2009 3]
[http://2009.igem.org/Team:Calgary/4_May_2009 4]	[http://2009.igem.org/Team:Calgary/5_May_2009 5]	[http://2009.igem.org/Team:Calgary/6_May_2009 6]	[http://2009.igem.org/Team:Calgary/7_May_2009 7]	[http://2009.igem.org/Team:Calgary/8_May_2009 8]	[http://2009.igem.org/Team:Calgary/9_May_2009 9]	[http://2009.igem.org/Team:Calgary/10_May_2009 10]
[http://2009.igem.org/Team:Calgary/11_May_2009 11]	[http://2009.igem.org/Team:Calgary/12_May_2009 12]	[http://2009.igem.org/Team:Calgary/13_May_2009 13]	[http://2009.igem.org/Team:Calgary/14_May_2009 14]	[http://2009.igem.org/Team:Calgary/15_May_2009 15]	[http://2009.igem.org/Team:Calgary/16_May_2009 16]	[http://2009.igem.org/Team:Calgary/17_May_2009 17]
[http://2009.igem.org/Team:Calgary/18_May_2009 18]	[http://2009.igem.org/Team:Calgary/19_May_2009 19]	[http://2009.igem.org/Team:Calgary/20_May_2009 20]	[http://2009.igem.org/Team:Calgary/21_May_2009 21]	[http://2009.igem.org/Team:Calgary/22_May_2009 22]	[http://2009.igem.org/Team:Calgary/23_May_2009 23]	[http://2009.igem.org/Team:Calgary/24_May_2009 24]
[http://2009.igem.org/Team:Calgary/25_May_2009 25]	[http://2009.igem.org/Team:Calgary/26_May_2009 26]	[http://2009.igem.org/Team:Calgary/27_May_2009 27]	[http://2009.igem.org/Team:Calgary/28_May_2009 28]	[http://2009.igem.org/Team:Calgary/29_May_2009 29]	[http://2009.igem.org/Team:Calgary/30_May_2009 30]	[http://2009.igem.org/Team:Calgary/31_May_2009 31]

June
M	T	W	T	F	S	S
[http://2009.igem.org/Team:Calgary/1_June_2009 1]	[http://2009.igem.org/Team:Calgary/2_June_2009 2]	[http://2009.igem.org/Team:Calgary/3_June_2009 3]	[http://2009.igem.org/Team:Calgary/4_June_2009 4]	[http://2009.igem.org/Team:Calgary/5_June_2009 5]	[http://2009.igem.org/Team:Calgary/6_June_2009 6]	[http://2009.igem.org/Team:Calgary/7_June_2009 7]
[http://2009.igem.org/Team:Calgary/8_June_2009 8]	[http://2009.igem.org/Team:Calgary/9_June_2009 9]	[http://2009.igem.org/Team:Calgary/10_June_2009 10]	[http://2009.igem.org/Team:Calgary/11_June_2009 11]	[http://2009.igem.org/Team:Calgary/12_June_2009 12]	[http://2009.igem.org/Team:Calgary/13_June_2009 13]	[http://2009.igem.org/Team:Calgary/14_June_2009 14]
[http://2009.igem.org/Team:Calgary/15_June_2009 15]	[http://2009.igem.org/Team:Calgary/16_June_2009 16]	[http://2009.igem.org/Team:Calgary/17_June_2009 17]	[http://2009.igem.org/Team:Calgary/18_June_2009 18]	[http://2009.igem.org/Team:Calgary/19_June_2009 19]	[http://2009.igem.org/Team:Calgary/20_June_2009 20]	[http://2009.igem.org/Team:Calgary/21_June_2009 21]
[http://2009.igem.org/Team:Calgary/22_June_2009 22]	[http://2009.igem.org/Team:Calgary/23_June_2009 23]	[http://2009.igem.org/Team:Calgary/24_June_2009 24]	[http://2009.igem.org/Team:Calgary/25_June_2009 25]	[http://2009.igem.org/Team:Calgary/26_June_2009 26]	[http://2009.igem.org/Team:Calgary/27_June_2009 27]	[http://2009.igem.org/Team:Calgary/28_June_2009 28]
[http://2009.igem.org/Team:Calgary/29_June_2009 29]	[http://2009.igem.org/Team:Calgary/30_June_2009 30]

July
M	T	W	T	F	S	S
		[http://2009.igem.org/Team:Calgary/1_July_2009 1]	[http://2009.igem.org/Team:Calgary/2_July_2009 2]	[http://2009.igem.org/Team:Calgary/3_July_2009 3]	[http://2009.igem.org/Team:Calgary/4_July_2009 4]	[http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6]	[http://2009.igem.org/Team:Calgary/7_July_2009 7]	[http://2009.igem.org/Team:Calgary/8_July_2009 8]	[http://2009.igem.org/Team:Calgary/9_July_2009 9]	[http://2009.igem.org/Team:Calgary/10_July_2009 10]	[http://2009.igem.org/Team:Calgary/11_July_2009 11]	[http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13]	[http://2009.igem.org/Team:Calgary/14_July_2009 14]	[http://2009.igem.org/Team:Calgary/15_July_2009 15]	[http://2009.igem.org/Team:Calgary/16_July_2009 16]	[http://2009.igem.org/Team:Calgary/17_July_2009 17]	[http://2009.igem.org/Team:Calgary/18_July_2009 18]	[http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20]	[http://2009.igem.org/Team:Calgary/21_July_2009 21]	[http://2009.igem.org/Team:Calgary/22_July_2009 22]	[http://2009.igem.org/Team:Calgary/23_July_2009 23]	[http://2009.igem.org/Team:Calgary/24_July_2009 24]	[http://2009.igem.org/Team:Calgary/25_July_2009 25]	[http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27]	[http://2009.igem.org/Team:Calgary/28_July_2009 28]	[http://2009.igem.org/Team:Calgary/29_July_2009 29]	[http://2009.igem.org/Team:Calgary/30_July_2009 30]	[http://2009.igem.org/Team:Calgary/31_July_2009 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/Team:Calgary/1_August_2009 1]	[http://2009.igem.org/Team:Calgary/2_August_2009 2]
[http://2009.igem.org/Team:Calgary/3_August_2009 3]	[http://2009.igem.org/Team:Calgary/4_August_2009 4]	[http://2009.igem.org/Team:Calgary/5_August_2009 5]	[http://2009.igem.org/Team:Calgary/6_August_2009 6]	[http://2009.igem.org/Team:Calgary/7_August_2009 7]	[http://2009.igem.org/Team:Calgary/8_August_2009 8]	[http://2009.igem.org/Team:Calgary/9_August_2009 9]
[http://2009.igem.org/Team:Calgary/10_August_2009 10]	[http://2009.igem.org/Team:Calgary/11_August_2009 11]	[http://2009.igem.org/Team:Calgary/12_August_2009 12]	[http://2009.igem.org/Team:Calgary/13_August_2009 13]	[http://2009.igem.org/Team:Calgary/14_August_2009 14]	[http://2009.igem.org/Team:Calgary/15_August_2009 15]	[http://2009.igem.org/Team:Calgary/16_August_2009 16]
[http://2009.igem.org/Team:Calgary/17_August_2009 17]	[http://2009.igem.org/Team:Calgary/18_August_2009 18]	[http://2009.igem.org/Team:Calgary/19_August_2009 19]	[http://2009.igem.org/Team:Calgary/20_August_2009 20]	[http://2009.igem.org/Team:Calgary/21_August_2009 21]	[http://2009.igem.org/Team:Calgary/22_August_2009 22]	[http://2009.igem.org/Team:Calgary/23_August_2009 23]
[http://2009.igem.org/Team:Calgary/24_August_2009 24]	[http://2009.igem.org/Team:Calgary/25_August_2009 25]	[http://2009.igem.org/Team:Calgary/26_August_2009 26]	[http://2009.igem.org/Team:Calgary/27_August_2009 27]	[http://2009.igem.org/Team:Calgary/28_August_2009 28]	[http://2009.igem.org/Team:Calgary/29_August_2009 29]	[http://2009.igem.org/Team:Calgary/30_August_2009 30]
[http://2009.igem.org/Team:Calgary/31_August_2009 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/Team:Calgary/1_September_2009 1]	[http://2009.igem.org/Team:Calgary/2_September_2009 2]	[http://2009.igem.org/Team:Calgary/3_September_2009 3]	[http://2009.igem.org/Team:Calgary/4_September_2009 4]	[http://2009.igem.org/Team:Calgary/5_September_2009 5]	[http://2009.igem.org/Team:Calgary/6_September_2009 6]
[http://2009.igem.org/Team:Calgary/7_September_2009 7]	[http://2009.igem.org/Team:Calgary/8_September_2009 8]	[http://2009.igem.org/Team:Calgary/9_September_2009 9]	[http://2009.igem.org/Team:Calgary/10_September_2009 10]	[http://2009.igem.org/Team:Calgary/11_September_2009 11]	[http://2009.igem.org/Team:Calgary/12_September_2009 12]	[http://2009.igem.org/Team:Calgary/13_September_2009 13]
[http://2009.igem.org/Team:Calgary/14_September_2009 14]	[http://2009.igem.org/Team:Calgary/15_September_2009 15]	[http://2009.igem.org/Team:Calgary/16_September_2009 16]	[http://2009.igem.org/Team:Calgary/17_September_2009 17]	[http://2009.igem.org/Team:Calgary/18_September_2009 18]	[http://2009.igem.org/Team:Calgary/19_September_2009 19]	[http://2009.igem.org/Team:Calgary/20_September_2009 20]
[http://2009.igem.org/Team:Calgary/21_September_2009 21]	[http://2009.igem.org/Team:Calgary/22_September_2009 22]	[http://2009.igem.org/Team:Calgary/23_September_2009 23]	[http://2009.igem.org/Team:Calgary/24_September_2009 24]	[http://2009.igem.org/Team:Calgary/25_September_2009 25]	[http://2009.igem.org/Team:Calgary/26_September_2009 26]	[http://2009.igem.org/Team:Calgary/27_September_2009 27]
[http://2009.igem.org/Team:Calgary/28_September_2009 28]	[http://2009.igem.org/Team:Calgary/29_September_2009 29]	[http://2009.igem.org/Team:Calgary/30_September_2009 30]

October
M	T	W	T	F	S	S
			[http://2009.igem.org/Team:Calgary/1_October_2009 1]	[http://2009.igem.org/Team:Calgary/2_October_2009 2]	[http://2009.igem.org/Team:Calgary/3_October_2009 3]	[http://2009.igem.org/Team:Calgary/4_October_2009 4]
[http://2009.igem.org/Team:Calgary/5_October_2009 5]	[http://2009.igem.org/Team:Calgary/6_October_2009 6]	[http://2009.igem.org/Team:Calgary/7_October_2009 7]	[http://2009.igem.org/Team:Calgary/8_October_2009 8]	[http://2009.igem.org/Team:Calgary/9_October_2009 9]	[http://2009.igem.org/Team:Calgary/10_October_2009 10]	[http://2009.igem.org/Team:Calgary/11_October_2009 11]
[http://2009.igem.org/Team:Calgary/12_October_2009 12]	[http://2009.igem.org/Team:Calgary/13_October_2009 13]	[http://2009.igem.org/Team:Calgary/14_October_2009 14]	[http://2009.igem.org/Team:Calgary/15_October_2009 15]	[http://2009.igem.org/Team:Calgary/16_October_2009 16]	[http://2009.igem.org/Team:Calgary/17_October_2009 17]	[http://2009.igem.org/Team:Calgary/18_October_2009 18]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/19_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/20_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/21_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/22_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/23_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/24_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/25_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/26_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/27_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/28_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/29_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/30_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=Team:Calgary/31_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 31]

JULY 26, 2009

CAROL

Transformation of week-long Ligation of promoter library and vector pCS26

Promoter Library product (from primer extension PCR) was ligated with pCS26 vector for 9 days with T4 Invitrogen Ligase at room temperature (~21^oC). This product was transformed into both XL Gold Ultracompetent and Top 10 cells.
Grew these cells on LB plates with Kan resistance at 37^oC for 16 hours.

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 JULY 26, 2009
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-Writing Paper
+Transformation of week-long Ligation of promoter library and vector pCS26
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-<br>
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 </html>
-* Finished writing modeling paper that summarizes what we will be doing once the circuit is completed. The experiments should not take long to accomplished and these results can be tabulated in Matlab quickly.
+* Promoter Library product (from primer extension PCR) was ligated with pCS26 vector for 9 days with T4 Invitrogen Ligase at room temperature (~21<sup>o</sup>C). This product was transformed into both XL Gold Ultracompetent and Top 10 cells.
-* Plated the week-long ligation of the promoter library in both XL Gold Ultra competent cells and Top 10 cells, but there is no growth.
+* Grew these cells on LB plates with Kan resistance at 37<sup>o</sup>C for 16 hours.
-* A reason that we thought of that might have caused minimal colonies is the phosphatase step. The 32 primers that we have synthesized does not have 5' phosphates. If we phosphatase the vector, that might result in no ligation.
-* Re-started restriction enzyme digestion on vector pCS26 with XhoI and BamHI at 37<sup>o</sub>C for 4 hours. Then I ligated the promoter with the vector with Quick Ligase for 5 minutes. Then I transformed the plasmids into both XL Gold Ultracompetent cells and Top 10 cells. Incubate at 37<sub>o</sub>C for 16 hours.
-<html>
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-<a name="Chinmoyee"></a>
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-CHINMOYEE
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-<td>
-<a name="Emily"></a>
-<br>
-<div class="heading">
-EMILY
-</div>
-<br>
-<div class="heading">
-Descriptive Title of What You're Doing
-</div>
-<br>
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-</html>
-WIKI CODING HERE
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-<a name="Fahd"></a>
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-FAHD
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-<br>
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-Descriptive Title of What You're Doing
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-</html>
-WIKI CODING HERE
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-<a name="Iman"></a>
-<br>
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-IMAN
-</div>
-<br>
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-Descriptive Title of What You're Doing
-</div>
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-</html>
-WIKI CODING HERE
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-<a name="Jamie"></a>
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-JAMIE
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-Descriptive Title of What You're Doing
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-WIKI CODING HERE
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-<a name="Jeremy"></a>
-<br>
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-JEREMY
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-<br>
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-Descriptive Title of What You're Doing
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-</html>
-WIKI CODING HERE
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-</td>
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-<tr>
-<td>
-<a name="Katie"></a>
-<br>
-<div class="heading">
-KATIE
-</div>
-<br>
-<div class="heading">
-Descriptive Title of What You're Doing
-</div>
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-</html>
-WIKI CODING HERE
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-<a name="Kevin"></a>
-<br>
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-KEVIN
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-<br>
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-Descriptive of What You're Doing
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-WIKI CODING HERE
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-<a name="Mandy"></a>
-<br>
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-MANDY
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-WIKI CODING HERE
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-<a name="Patrick"></a>
-<br>
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-PATRICK
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-<br>
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-Descriptive Title of What You're Doing
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-WIKI CODING HERE
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-<td>
-<a name="Prima"></a>
-<br>
-<div class="heading">
-PRIMA
-</div>
-<br>
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-Descriptive Title of What You're Doing
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-</html>
-WIKI CODING HERE
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-<a name="Stefan"></a>
-<br>
-<div class="heading">
-STEFAN
-</div>
-<br>
-<div class="heading">
-Descriptive Title of What You're Doing
-</div>
-<br>
-<div class="desc">
-</html>
-WIKI CODING HERE
-<html>
-</div>
-</td>
-</tr>
-<tr>
-<td height="10px" bgcolor="#222222">
-</td>
-</tr>
-<tr>
-<td>
-<a name="Vicki"></a>
-<br>
-<div class="heading">
-VICKI
-</div>
-<br>
-<div class="heading">
-Descriptive Title of What You're Doing
-</div>
-<br>
-<div class="desc">
-</html>
-WIKI CODING HERE
-<html>
-</div>
-</td>
-</tr>
 </table>
 </body>
 </html>