CAROL
Transformation of week-long Ligation of promoter library and vector pCS26
- Promoter Library product (from primer extension PCR) was ligated with pCS26 vector for 9 days with T4 Invitrogen Ligase at room temperature (~21oC). This product was transformed into both XL Gold Ultracompetent and Top 10 cells.
- Grew these cells on LB plates with Kan resistance at 37oC for 16 hours.
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