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EPF-Lausanne/1 August 2009
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We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!! | We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!! | ||
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What was left of these clones was then put into liquid culture and left to incubate at 37°C. | What was left of these clones was then put into liquid culture and left to incubate at 37°C. | ||
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| + | At around 30 past midnight minipreps of these clones were done! | ||
==People in the lab== | ==People in the lab== | ||
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Latest revision as of 19:56, 2 August 2009
Contents |
Wet Lab
We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!!
What was left of these clones was then put into liquid culture and left to incubate at 37°C.
At around 30 past midnight minipreps of these clones were done!
People in the lab
Nath, Basile, Gab
