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Uppsala-Sweden/3 August 2009
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==Glycerol Stock for Z. Mobilis== | ==Glycerol Stock for Z. Mobilis== | ||
The test protocol works really fine as prelimary test results show. | The test protocol works really fine as prelimary test results show. | ||
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--[[User:Karl.brune|Karl.brune]] 12:42, 3 August 2009 (UTC) | --[[User:Karl.brune|Karl.brune]] 12:42, 3 August 2009 (UTC) | ||
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| + | ==Buffers for transformation== | ||
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| + | We prepared the buffers (TE and SOB) needed for the transformation. | ||
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| + | The protocols can be found here [http://openwetware.org/wiki/SOB] and [http://openwetware.org/wiki/TE_buffer] | ||
| + | --[[User:Flormane|Flormane]] 12:13, 4 August 2009 (UTC) | ||
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| + | ==PCR, Kivd, ADH2 and PpetE== | ||
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| + | We started a PCR reaction to extract the Kivd gene from Lactoctococcus Lactis sp.Lactis, ADH2 from Saccharomyces cerevisiae and the promoter PpetE from Synechocystis. | ||
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| + | The primers used was [[Media:primers_090803_adh2_kivd_ppete.xls]] | ||
| + | --[[User:Flormane|Flormane]] 12:13, 4 August 2009 (UTC) | ||
Latest revision as of 12:15, 4 August 2009
Glycerol Stock for Z. Mobilis
The test protocol works really fine as prelimary test results show. Thus I made final gylcerol stocks for Z. Mobilis.
The protocol can be found here: Media:Glycerol_Stock_Z_mobilis.xls
--Karl.brune 12:42, 3 August 2009 (UTC)
Buffers for transformation
We prepared the buffers (TE and SOB) needed for the transformation.
The protocols can be found here [http://openwetware.org/wiki/SOB] and [http://openwetware.org/wiki/TE_buffer] --Flormane 12:13, 4 August 2009 (UTC)
PCR, Kivd, ADH2 and PpetE
We started a PCR reaction to extract the Kivd gene from Lactoctococcus Lactis sp.Lactis, ADH2 from Saccharomyces cerevisiae and the promoter PpetE from Synechocystis.
The primers used was Media:primers_090803_adh2_kivd_ppete.xls --Flormane 12:13, 4 August 2009 (UTC)
