Team:Valencia/Notebook/July

From 2009.igem.org

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| 13 ||[[Team:Valencia/Notebook/July#July 14th|14]] || [[Team:Valencia/Notebook/July#July 15th|15]] || &nbsp;[[Team:Valencia/Notebook/July#July 16th|16]] || [[Team:Valencia/Notebook/July#July 17th|17]] || [[Team:Valencia/Notebook/July#July 18th|18]] || 19 ||  
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</div>
</div>
-
=== July 1st ===
+
=== July 28th ===
-
- We prepared some [[Team:Valencia/Notebook/Protocols#Glycerol Stock | glycerol stock]] from the four ''Saccharomyces cerevisiae'' strains we received from [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6 Eduardo Rial]  : <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Negative control, '''UCP -'''. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Strain containing UCP1, '''UCP +'''. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Mutant stains over expressing UCP1, '''Gly 76Δ''' and '''Gly 175Δ'''. <br>
+
 +
Today we have prepared the SD medium lacking Leu that we need for the yeast experiment. We have 500 ml  of solid medium and 500 ml of liquid medium. That should be more than enough for all the summer.
 +
The components of the medium are:
-
- We calibrated our calorimeters with hot water.
 
-
 
-
=== July 2nd ===
 
-
- We prepared our first [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium.]]
 
-
 
-
- We calibrated our calorimeters with hot water.
 
-
 
-
=== July 4th ===
 
-
- We prepared some plates for inoculum. They were stored a the 28ºC stove.
 
-
 
-
=== July 7th ===
 
-
- We prepared some inoculum in Erlenmeyer flasks for our experiment the following day. We kept them overnight in a 30ºC shaking stove.
 
-
 
-
=== July 8th ===
 
-
- The experiment consisted on: <br>
 
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br>
 
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains <br>
 
-
We left it overnight, but the air conditioning went off and the room temperature rose. 
 
-
 
-
===July 9th===
 
-
- We characterized culture growth in our calorimeters by measuring DO, with just one strain.<br>
 
-
 
-
===July 10th===
 
-
- Trying to see if there is basal rate of UCP1. Experiment: <br>
 
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SD Medium | SD medium]] <br>
 
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Calorimeters containing: water, medium, UCP-, Gly 175Δ.
 
-
 
-
===July 11th===
 
-
- It looks like our ''Saccharomyces cerevisiae'' mutant strain has a basal rate of UCP1... We repeated the experiment to be sure.
 
-
 
-
===July 12th===
 
-
- We obtained the same results.
 
-
 
-
===July 14th===
 
-
- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.
 
-
 
-
===July 15th===
 
-
- We prepared inoculum in Erlenmeyer flasks. We kept them overnight in a 30ºC shaking stove.
 
   
   
-
===July 16th===
+
- 0.5% (NH4)2SO4
-
- Trying to find out which is the best moment for activating UCP1.... The glucose in the medium must be consumed by the induction time. DO at the beginning of the experiment was 0.05. <br>
+
-
Experiment: <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]] <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Galactose activation. <br>
+
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; One strain. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Activation at two, three and four hours after inoculating. D.O. was measured at each time.<br>
+
-
===July 17th===
+
- 0.17% Yeast Synthetic Drop-Out Medium Supplement Without Leucine
-
- Our lab electricity went off during the night. How many experiments will have it ruined?
+
-
===July 18th===
+
- 2% Glucose
-
- We prepared lots of plates with our [[Team:Valencia/Notebook/Protocols#SD Medium |SD medium]]...Unfortunately, we forgot to add glucose.
+
-
- We carried out the pilot experiment: <br>
+
- 1'5% Agar (only for the solid medium)
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Protocols#SP liquid medium |SP medium]]. <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Galactose activation at 3 hours (approximate DO 0.15). <br>
+
-
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains. <br>
+
-
We see weird oscillations.
+
-
===July 20th===
 
-
- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.
 
-
===July 21st===
 
-
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] from July 18th again. Weird results, we need to repeat it.
 
-
===July 22nd===
+
<br><br><br><br><br><br>
-
- We repaired our calorimeters. They were the reason for yesterday weird results.
+
-
===July 23rd===
+
<center><html>
-
- We repeated the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] from July 18th.
+
<div style=" width:50%;">
-
 
+
<h2>
-
===July 24th===
+
Back to<a href="https://2009.igem.org/Team:Valencia/Notebook/June"><font color="#047DB5"> June </font></a>&nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;July &nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;Go to <a href="https://2009.igem.org/Team:Valencia/Notebook/August"><font color="#047DB5"> August </font></a></h2>
-
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste... They wanted to find out if the oscillations would stop if they covered the thermocouples with different materials they found in the contiguuos labs, or immerse them in the liquid. <br>
+
</div>
-
Meri, who is in charged of the lab, wasn't very happy the following day...
+
</html></center>
-
 
+
-
===July 25th===
+
-
- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.
+
-
 
+
-
===July 26th===
+
-
- One of our calorimeters wasn't working properly.
+
-
 
+
-
===July 28th===
+
-
- We repaired the calorimeter and calibrated once again.
+
-
 
+
-
===July 29th===
+
-
- Our cultures haven't grown enough for the experiment. Temperature and medium were all right, we'll have to wait...
+
-
 
+
-
===July 30th===
+
-
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]], same as July 18th. Weird results...
+
-
 
+
-
===July 31st===
+
-
- We carried out the [[Team:Valencia/Notebook/July#July 18th | pilot experiment]] once again.
+

Latest revision as of 07:22, 20 October 2009




April
Mo Tu We Th Fr Sa Su
30 31  1  2  3  4  5
 6  7  8  9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30  1  2  3
May
Mo Tu We Th Fr Sa Su
27 28 29 30  1  2  3
 4  5  6  7  8  9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
Mo Tu We Th Fr Sa Su
 1  2  3  4  5  6  7
 8  9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30  1   2  3  4  5
July
Mo Tu We Th Fr Sa Su
29 30  1  2  3  4  5
 6  7  8  9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31   1  2
August
Mo Tu We Th Fr Sa Su
27 28 29  30 31  1  2
 3  4  5  6  7  8  9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31 1 2 3 4 5 6
September
Mo Tu We Th Fr Sa Su
31  1  2  3  4  5  6
 7  8  9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30  1 2 3 4
October
Mo Tu We Th Fr Sa Su
28 29 30   1  2  3  4
 5  6  7  8  9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31  1
November
Mo Tu We Th Fr Sa Su
26 27 28  29 30 31  1
 2  3  4  5  6  7  8
 9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30  1  2  3  4  5  6

July 28th

Today we have prepared the SD medium lacking Leu that we need for the yeast experiment. We have 500 ml of solid medium and 500 ml of liquid medium. That should be more than enough for all the summer. The components of the medium are:


- 0.5% (NH4)2SO4

- 0.17% Yeast Synthetic Drop-Out Medium Supplement Without Leucine

- 2% Glucose

- 1'5% Agar (only for the solid medium)








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