Virginia Commonwealth/12 June 2009
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==Friday 12 June 2009== | ==Friday 12 June 2009== | ||
===Results=== | ===Results=== | ||
- | *Data from plasmid purification | + | *Data from plasmid purification (Miniprep) |
**Buffer EB was used instead of water and 500 microliters was used instead of 50 microliters so the DNA will have to be concentrated. | **Buffer EB was used instead of water and 500 microliters was used instead of 50 microliters so the DNA will have to be concentrated. | ||
- | **114 microliters of buffer and 6 microliters of sample | + | **114 microliters of buffer and 6 microliters of sample (20x dilution) |
{| cellpadding="10" align="center" border="1" padding="5" | {| cellpadding="10" align="center" border="1" padding="5" | ||
|+ | |+ | ||
|- | |- | ||
- | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! | + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Conc. (µg/mL) !! Amount (µg) !! Volume (µL) |
|- | |- | ||
! J23103 | ! J23103 | ||
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|} | |} | ||
- | + | *100 microliters of buffer and 20 microliters of sample (6x dilution) | |
{| cellpadding="10" align="center" border="1" padding="5" | {| cellpadding="10" align="center" border="1" padding="5" | ||
|+ | |+ | ||
|- | |- | ||
- | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Concentration ( | + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Concentration (µg/mL) !! Amount (µg) !! Volume (µL) |
|- | |- | ||
! J23102 | ! J23102 | ||
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===Tasks=== | ===Tasks=== | ||
- | |||
*Review Literature | *Review Literature | ||
- | + | ---- | |
+ | |||
====Wetlab==== | ====Wetlab==== | ||
*Mini-Prep on the promoter. | *Mini-Prep on the promoter. | ||
*Maxi-Prep on the reporter and the backbone. | *Maxi-Prep on the reporter and the backbone. | ||
- | |||
**Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing. | **Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing. | ||
- | + | [[User:MandM|MandM]] 19:01, 12 June 2009 (UTC) | |
+ | |||
+ | [[User:Bussingkm|Bussingkm]] 15:40, 13 June 2009 (UTC) |
Latest revision as of 16:04, 5 August 2009
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Contents |
Friday 12 June 2009
Results
- Data from plasmid purification (Miniprep)
- Buffer EB was used instead of water and 500 microliters was used instead of 50 microliters so the DNA will have to be concentrated.
- 114 microliters of buffer and 6 microliters of sample (20x dilution)
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Conc. (µg/mL) | Amount (µg) | Volume (µL) |
---|---|---|---|---|---|---|
J23103 | .007 | .003 | 2.33 | 7.0 | 2.52 | 360 |
J23105 | .008 | .004 | 2.00 | 8.0 | 2.96 | 370 |
J23100 | .014 | .011 | 1.273 | 14.0 | 5.32 | 380 |
J23104 | .011 | .009 | 1.22 | 11.0 | 4.18 | 380 |
J23101 | .012 | .008 | 1.5 | 12.0 | 4.56 | 380 |
J23107 | .018 | .014 | 1.286 | 18.0 | 8.1 | 450 |
J23116 | .020 | .016 | 1.25 | 20.0 | 7.2 | 360 |
J23113 | .014 | .010 | 1.4 | 14.0 | 5.18 | 370 |
J23110 | .012 | .007 | 1.714 | 12.0 | 4.2 | 350 |
- 100 microliters of buffer and 20 microliters of sample (6x dilution)
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Concentration (µg/mL) | Amount (µg) | Volume (µL) |
---|---|---|---|---|---|---|
J23102 | .031 | .025 | 1.24 | 31.0 | 10.23 | 330 |
R0011 | .021 | .016 | 1.313 | 21.0 | 5.46 | 260 |
R0040 | .015 | .011 | 1.364 | 15.0 | 5.4 | 360 |
MandM 19:17, 12 June 2009 (UTC)
Tasks
- Review Literature
Wetlab
- Mini-Prep on the promoter.
- Maxi-Prep on the reporter and the backbone.
- Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing.
MandM 19:01, 12 June 2009 (UTC)
Bussingkm 15:40, 13 June 2009 (UTC)