Template:Team:KULeuven/5 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)

Latest revision as of 09:49, 14 September 2009

Miniprep of pairs:

RBS + sam5 conc: 101,3 ng/ul
RBS + sam8 conc: 95.7 ng/ul
sam8 conc: 81,8 ng/ul
COMT , conc: 122,9 ng/ul

Restriction digestion
A1: sam8, EcoRI en SpeI
A2: sam5, EcoRI en XbaI

B1: RBS, EcoRI en SpeI
B2: COMT, EcoRI en XbaI

C1: fcs, EcoRI en SpeI
C2: ech, EcoRI en XbaI

The mixture was incubated in 37°C for 1h30...

...followed by gel electrophoresis of the digested parts

@@ Line 1: / Line 1: @@
 Miniprep of pairs:
-* RBS + Sam5 {{kulpart|K238007}} conc: 101,3 ng/ul
+* RBS + ''sam5'' {{kulpart|BBa_K238007}} conc: 101,3 ng/ul
-* RBS+Sam8 {{kulpart|I742106}} conc: 95.7 ng/ul
+* RBS + ''sam8'' {{kulpart|BBa_I742106}} conc: 95.7 ng/ul
-* Sam8 {{kulpart|I742142}} conc: 81,8 ng/ul
+* ''sam8'' {{kulpart|BBa_I742142}} conc: 81,8 ng/ul
-* ComT {{kulpart|I742107}}, conc: 122,9 ng/ul
+* ''COMT'' {{kulpart|BBa_I742107}}, conc: 122,9 ng/ul
-Restriction digestion
+Restriction digestion<br>
-A1: Sam8, EcoRI en SpeI <br>
+A1: '''''sam8''''', EcoRI en SpeI <br>
-A2: Sam5, EcoRI en XbaI<br>
+A2: '''''sam5''''', EcoRI en XbaI<br>
 <br>
-B1: RBS, EcoRI en SpeI<br>
+B1: '''RBS''', EcoRI en SpeI<br>
-B2: ComT, EcoRI en XbaI<br>
+B2: '''''COMT''''', EcoRI en XbaI<br>
 <br>
-C1: Fcs, EcoRI en SpeI <br>
+C1: '''''fcs''''', EcoRI en SpeI <br>
-C2: Ech, EcoRI en XbaI<br>
+C2: '''''ech''''', EcoRI en XbaI<br>
-The mixture was incubated in 37C for 1h30
+The mixture was incubated in 37°C for 1h30...
-Followed by gelelectroforesis of the digested parts
+...followed by gel electrophoresis of the digested parts