Virginia Commonwealth/11 August 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 16:29, 13 August 2009

May
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				[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14]	[http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23]	[http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]

June
M	T	W	T	F	S	S
[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]

July
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		[http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30]	[http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]

August
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					[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15]	[http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]

September
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	[http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]

October
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			[http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Tuesday 11 August 2009

Results

Kevin and Adam

I have cell growth on all plates. Yesterday's work was successful. Colonies number from 2-5 colonies per plate at 4:00 PM. I will wait until later to pick a colony.

Maria and Afton

1kb ladder has not come in yet so, digests and minipreps will be run on gels when it comes in
Plated colonies from Friday (8/7/09) transformations are strongly espressing RFP
- It might be better to pick colonies 3 to 4 days after transformation when colonies are strongly espressing RFP, instead of when the e. coli are pink the day after transformation
Spectrophotometry Data


Part No.	A260 (nm)	A280 (nm)	A260/A280	Vol. (µL)	Conc. (µg/mL)	Amt (µg)
pSB1C3 p1010	0.012	0.008	1.5	54	24	1.3
J23100	0.029	0.023	1.26	35	58	2.03
J23104	0.030	0.026	1.15	38	60	2.28
J23102	0.022	0.019	1.16	40	44	1.76
J23103	0.021	0.015	1.40	45	42	1.89
J06702	0.029	0.018	1.61	117	58	6.79

Trentay 19:17, 11 August 2009 (UTC)

Tasks

Maria and Afton

Take Spectrophotometry measurements of pSB1C3 p1010, J06702, J23100, J23102, J23103, J23104
- Need to locate J23100 to be digested another day
Digest above parts
Ligate J06702 and pSB1C3 with the following promoters: 100, 102, 103, 104
Transform parts into NEB10β
Update Wiki
Make overnight cultures of I1352/pSB1C3
- I1352/I1352 will not be kept because it never expressed RFP and there are plenty of stock of I1352 with Amp resistance
Make overnight cultures of pSB1C3 p1010 from cryogenic storage to Miniprep tomorrow
Make more LB+Cm plates

Trentay 14:43, 11 August 2009 (UTC)

Wetlab

Maria and Afton

Spectrophotometry was done
Digest was done
Ligation was done
Transformation was done
Overnight cultures were made
LB+Cm plates were made

Trentay 20:12, 11 August 2009 (UTC)

Adam and Kevin

The growth on the plates remains slow. No colonies show fluorescence yet, for the sake of better selection I am going to wait until early tomorrow morning to pick colonies. I will cultivate them tomorrow and then store them in the evening. Hopefully we will be able to measure transcription levels Thursday.

-Bussingkm 21:16, 11 August 2009 (UTC)

@@ Line 39: / Line 39: @@
 ==Tuesday 11 August 2009==
 ===Results===
-* 1
+''Kevin and Adam''
-* 2
+*I have cell growth on all plates.  Yesterday's work was successful.  Colonies number from 2-5 colonies per plate at 4:00 PM.  I will wait until later to pick a colony.
+''Maria and Afton''
+* 1kb ladder has not come in yet so, digests and minipreps will be run on gels when it comes in
+* Plated colonies from Friday (8/7/09) transformations are strongly espressing RFP
+** It might be better to pick colonies 3 to 4 days after transformation when colonies are strongly espressing RFP, instead of when the e. coli are pink the day after transformation
+*Spectrophotometry Data
+{| cellpadding="5" border="1" padding="5"
+|+
+|-
+! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !!  Vol. (µL) !! Conc. (µg/mL) !! Amt (µg)
+|-
+| pSB1C3 p1010 || 0.012 || 0.008 || 1.5 || 54 || 24 || 1.3
+|-
+| J23100 || 0.029 || 0.023 || 1.26 || 35 || 58 || 2.03
+|-
+| J23104 || 0.030 || 0.026 || 1.15 || 38 || 60 || 2.28
+|-
+| J23102 || 0.022 || 0.019 || 1.16 || 40 || 44 || 1.76
+|-
+| J23103 || 0.021 || 0.015 || 1.40 || 45 || 42 || 1.89
+|-
+| J06702 || 0.029 || 0.018 || 1.61 || 117 || 58 || 6.79
+|}
+[[User:Trentay|Trentay]] 19:17, 11 August 2009 (UTC)
 ----
 ===Tasks===
-* 1
+''Maria and Afton''
-* 2
+* Take Spectrophotometry measurements of pSB1C3 p1010, J06702, J23100, J23102, J23103, J23104
+** Need to locate J23100 to be digested another day
+* Digest above parts
+* Ligate J06702 and pSB1C3 with the following promoters: 100, 102, 103, 104
+* Transform parts into NEB10β
+* Update Wiki
+* Make overnight cultures of I1352/pSB1C3
+** I1352/I1352 will not be kept because it never expressed RFP and there are plenty of stock of I1352 with Amp resistance
+* Make overnight cultures of pSB1C3 p1010 from cryogenic storage to Miniprep tomorrow
+* Make more LB+Cm plates
+[[User:Trentay|Trentay]] 14:43, 11 August 2009 (UTC)
 ----
 ====Wetlab====
-* 1
+''Maria and Afton''
-* 2
+* Spectrophotometry was done
+* Digest was done
+* Ligation was done
+* Transformation was done
+* Overnight cultures were made
+* LB+Cm plates were made
+[[User:Trentay|Trentay]] 20:12, 11 August 2009 (UTC)
+''Adam and Kevin''
+* The growth on the plates remains slow.  No colonies show fluorescence yet, for the sake of better selection I am going to wait until early tomorrow morning to pick colonies.  I will cultivate them tomorrow and then store them in the evening.  Hopefully we will be able to measure transcription levels Thursday.
+-[[User:Bussingkm|Bussingkm]] 21:16, 11 August 2009 (UTC)

Virginia Commonwealth/11 August 2009

From 2009.igem.org

Latest revision as of 16:29, 13 August 2009

Contents

Tuesday 11 August 2009

Results

Tasks

Wetlab