Virginia Commonwealth/11 August 2009

Tuesday 11 August 2009

Kevin and Adam

I have cell growth on all plates. Yesterday's work was successful. Colonies number from 2-5 colonies per plate at 4:00 PM. I will wait until later to pick a colony.

Maria and Afton

1kb ladder has not come in yet so, digests and minipreps will be run on gels when it comes in
Plated colonies from Friday (8/7/09) transformations are strongly espressing RFP
- It might be better to pick colonies 3 to 4 days after transformation when colonies are strongly espressing RFP, instead of when the e. coli are pink the day after transformation
Spectrophotometry Data


Part No.	A260 (nm)	A280 (nm)	A260/A280	Vol. (µL)	Conc. (µg/mL)	Amt (µg)
pSB1C3 p1010	0.012	0.008	1.5	54	24	1.3
J23100	0.029	0.023	1.26	35	58	2.03
J23104	0.030	0.026	1.15	38	60	2.28
J23102	0.022	0.019	1.16	40	44	1.76
J23103	0.021	0.015	1.40	45	42	1.89
J06702	0.029	0.018	1.61	117	58	6.79

Trentay 19:17, 11 August 2009 (UTC)

Maria and Afton

Take Spectrophotometry measurements of pSB1C3 p1010, J06702, J23100, J23102, J23103, J23104
- Need to locate J23100 to be digested another day
Digest above parts
Ligate J06702 and pSB1C3 with the following promoters: 100, 102, 103, 104
Transform parts into NEB10β
Update Wiki
Make overnight cultures of I1352/pSB1C3
- I1352/I1352 will not be kept because it never expressed RFP and there are plenty of stock of I1352 with Amp resistance
Make overnight cultures of pSB1C3 p1010 from cryogenic storage to Miniprep tomorrow
Make more LB+Cm plates

Trentay 14:43, 11 August 2009 (UTC)

Maria and Afton

Trentay 20:12, 11 August 2009 (UTC)

Adam and Kevin

The growth on the plates remains slow. No colonies show fluorescence yet, for the sake of better selection I am going to wait until early tomorrow morning to pick colonies. I will cultivate them tomorrow and then store them in the evening. Hopefully we will be able to measure transcription levels Thursday.

-Bussingkm 21:16, 11 August 2009 (UTC)