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Uppsala-Sweden/7 August 2009
From 2009.igem.org
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Karl.brune (Talk | contribs) (New page: {{Uppsala-Sweden Template}} ==Overview sheet== Media:Example.ogg ==Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3== Ligation with the pSB1A3 vector using the Quick Ligat...) |
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==Overview sheet== | ==Overview sheet== | ||
| - | [[Media: | + | [[Media:Transformation Workorder.pdf]] |
==Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3== | ==Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3== | ||
Ligation with the pSB1A3 vector using the Quick Ligation Kit from [http://www.neb.com/nebecomm/products/protocol2.asp NEB]. | Ligation with the pSB1A3 vector using the Quick Ligation Kit from [http://www.neb.com/nebecomm/products/protocol2.asp NEB]. | ||
| - | Calculated amounts can be found here [[Media:Ligation Calc 07082009.xls] | + | Calculated amounts can be found here [[Media:Ligation Calc 07082009.xls]] |
==Transformation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into competent E. coli cells== | ==Transformation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into competent E. coli cells== | ||
Transformation was performed as written in the protocol form the Ligation kit from [http://www.neb.com/nebecomm/products/protocol3.asp NEB] | Transformation was performed as written in the protocol form the Ligation kit from [http://www.neb.com/nebecomm/products/protocol3.asp NEB] | ||
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--[[User:Karl.brune|Karl.brune]] 08:56, 10 August 2009 (UTC) | --[[User:Karl.brune|Karl.brune]] 08:56, 10 August 2009 (UTC) | ||
Latest revision as of 09:30, 10 August 2009
Overview sheet
Media:Transformation Workorder.pdf
Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3
Ligation with the pSB1A3 vector using the Quick Ligation Kit from [http://www.neb.com/nebecomm/products/protocol2.asp NEB]. Calculated amounts can be found here Media:Ligation Calc 07082009.xls
Transformation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into competent E. coli cells
Transformation was performed as written in the protocol form the Ligation kit from [http://www.neb.com/nebecomm/products/protocol3.asp NEB]
--Karl.brune 08:56, 10 August 2009 (UTC)
