Virginia Commonwealth/12 August 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 18:33, 13 August 2009

May
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				[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14]	[http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23]	[http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]

June
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[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]

July
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		[http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30]	[http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]

August
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					[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15]	[http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]

September
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	[http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]

October
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			[http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Wednesday 12 August 2009

Results

**growth results**
Construct	Growth	Flourescence!
D1+BBa_J23101+pSB1C3	No Growth	N/A
D2+BBa_J06702+pSB1C3	No Growth	N/A
D3+BBa_J06702+pSB1C3	One Colony	Minimal at Best
D4+BBa_J06702+pSB1C3	No Growth	N/A
D5+BBa_J06702+pSB1C3	Three Colonies	No Flourescence
D6+BBa_J06702+pSB1C3	One Colony	No Flourescence
D7+BBa_J06702+pSB1C3	One Colony	Possible
D8+BBa_J06702+pSB1C3	No Growth	N/A
D9+BBa_J06702+pSB1C3	Three Colonies	No Flourescence
Positive Control	Lawn	No Flourescence
Negative Control	No Growth	N/A

I will wait for fluorescence to pick colonies.

Maria and Afton

Results of transformation were not good


Name	Plate	Growth Observation
J23100 w/1C3,702	Cm	no growth
J23102 w/1C3,702	Cm	no growth
J23103 w/1C3,702	Cm	no growth
J23104 w/1C3,702	Cm	no growth

A gel will be run today on the digests to see how they look.
Plates will be kept for a few more days in case the bacteria are growing slowly

Trentay 17:55, 12 August 2009 (UTC)

Tasks

Maria and Afton

Miniprep more pSB1C3 p1010
Take Spectrophotometry measurements for J06702, pSB1C3, 101, 105, 106, 107
Digest promoters 101, 105, 106, 107 along with pSB1C3, J06702
Run a gel on the digests
- Everything fit on the gel except for 106 and 107

Trentay 17:55, 12 August 2009 (UTC)

Wetlab

Kevin and Adam

I don't think that the no growth constructs were not successful. The last ligation reaction cleaned out the PCR product for D1-9. Will perform another PCR reaction today so the ligation can be done again.
- Purified PCR product did not show up on gel. I will redo reaction tomorrow. Bussingkm 22:26, 12 August 2009 (UTC)

Maria and Afton

Miniprep was done
Spectrophotometry was done
Digest was done
Gel was run

Trentay 19:44, 12 August 2009 (UTC)

@@ Line 68: / Line 68: @@
 I will wait for fluorescence to pick colonies.
+''Maria and Afton''
+* Results of transformation were not good
+{| cellpadding="5" border="1" padding="5"
+|+
+|-
+! Name !! Plate !! Growth Observation
+|-
+| J23100 w/1C3,702 || Cm || no growth
+|-
+| J23102 w/1C3,702 || Cm || no growth
+|-
+| J23103 w/1C3,702 || Cm || no growth
+|-
+| J23104 w/1C3,702 || Cm || no growth
+|}
+* A gel will be run today on the digests to see how they look.
+* Plates will be kept for a few more days in case the bacteria are growing slowly
+[[User:Trentay|Trentay]] 17:55, 12 August 2009 (UTC)
 ----
 ===Tasks===
-* 1
+''Maria and Afton''
-* 2
+* Miniprep more pSB1C3 p1010
+* Take Spectrophotometry measurements for J06702, pSB1C3, 101, 105, 106, 107
+* Digest promoters 101, 105, 106, 107 along with pSB1C3, J06702
+* Run a gel on the digests
+** Everything fit on the gel except for 106 and 107
+[[User:Trentay|Trentay]] 17:55, 12 August 2009 (UTC)
 ----
 ====Wetlab====
-* 1 I don't think that the no growth constructs were not successful.  The last ligation reaction cleaned out the PCR product for D1-9.  Will perform another PCR reaction today so the ligation can be done again.
+''Kevin and Adam''
-* 2
+* I don't think that the no growth constructs were not successful.  The last ligation reaction cleaned out the PCR product for D1-9.  Will perform another PCR reaction today so the ligation can be done again.
+** Purified PCR product did not show up on gel.  I will redo reaction tomorrow. [[User:Bussingkm|Bussingkm]] 22:26, 12 August 2009 (UTC)
+''Maria and Afton''
+* Miniprep was done
+* Spectrophotometry was done
+* Digest was done
+* Gel was run
+[[User:Trentay|Trentay]] 19:44, 12 August 2009 (UTC)

Virginia Commonwealth/12 August 2009

From 2009.igem.org

Latest revision as of 18:33, 13 August 2009

Contents

Wednesday 12 August 2009

Results

Tasks

Wetlab