From 2009.igem.org
(Difference between revisions)
|
|
(3 intermediate revisions not shown) |
Line 62: |
Line 62: |
| ===Transporters=== | | ===Transporters=== |
| | | |
| + | [[Image:F102471_2009-08-14_12hr_03min_PCR1_PCR2_Dynazyme_XbaI.jpg|thumb|PCR2, PCR1 Dynazyme XbaI]] |
| + | ====HmtA==== |
| After 2 weeks of PCR-ing plasmid isolations there is a product worth wile to send for sequencing. 4,5,6, and 8 | | After 2 weeks of PCR-ing plasmid isolations there is a product worth wile to send for sequencing. 4,5,6, and 8 |
| | | |
Line 67: |
Line 69: |
| | | |
| In the lower left quarter is a PCR on HmtA-AC3 plasmid with PstI 's left in place with VF2 VR and failed. | | In the lower left quarter is a PCR on HmtA-AC3 plasmid with PstI 's left in place with VF2 VR and failed. |
- | The second lower quarter is a XbaI restriction control on plasmids 4,5,6 and 8 not completely digested but indicating the construct is correct. | + | The second lower quarter is a XbaI restriction control on plasmids 4,5,6 and 8 not completely digested but indicating the construct is correct in the way that there is a restriction site of the bio brick, meaning the EcoRI mutation got removed. |
- | [[Image:F102471_2009-08-14_12hr_03min_PCR1_PCR2_Dynazyme_XbaI.jpg|thumb|PCR2, PCR1 Dynazyme XbaI]]
| + | |
| | | |
| ===Metal Accumulation=== | | ===Metal Accumulation=== |
Line 79: |
Line 80: |
| | | |
| ==Dry== | | ==Dry== |
| + | Jasper changed [[Team:Groningen/Modelling/Arsenic|the model]] to take dimeric binding of ArsR into account and to ignore ArsD, as well as putting an image map on-line as the first step towards a clearer interface. The new model leads to a number of third order equations, which means that it is no longer feasible to give closed-form solutions, but it does seem perfectly feasible to compute the solutions numerically (as demonstrated by the updated calculator on the [[Team:Groningen/Project/Accumulation|accumulation page]]). |
| | | |
| {{Team:Groningen/Notebook/Day/Footer}} | | {{Team:Groningen/Notebook/Day/Footer}} |
Latest revision as of 06:49, 17 August 2009
Wet
GVP Cluster
- → DONE make glycerol stocks for pSB1AC3-BBa_J23106-BBa_I750016 and pSB1AC3-BBa_J23109-BBa_I750016 (two o.n. cultures of each are grown)
- → DONE isolate plasmid J61002-BBa_J23100, J61002-BBa_J23106, and J61002-BBa_J23109 from o.n. cultures
- → DONE make a planning for next week
Plates
Showed single colony growth on plates with pSB3K3-H/L-GVP plasmids, and were stored in the fridge for future preculture growth.
- → The plates with pSB1AC3-H-GVP plasmid showed no growth, and were stored at 37°C for an additional 4 hours to be sure. Still no growth after addition incubation at 37°C, can be due to high energy cost of vesicle production resulting in the death of the cells!
Over Night Cultures
- → All cultures showed growth, and can be used for plasmid isolation and glycerol stocks. From each culture 800uL was added to 300uL 87% glycerol and frozen in liquid nitrogen. Stocks were stored at position 21 to 24 in the -80°C fridge.
Plasmid Purification
Plasmid isolation was performed on the cultures of E.coli TOP10 containing plasmids [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] with [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 high], [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 medium] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23109 low] constitutive promoters with the "Sygma-Aldrich™ [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/plasmid-miniprep-kit.html GenElute™] Plasmid Miniprep Kit".
- From each tube 8mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Plasmids were eluted with 50μL MQ and stored in the fridge
Concentration
Plasmid
| Conc. ng/μL
| 260/280
| 260/230
| -20 box (michael
| Restriction Control
|
BBa_J61002-BBa_J23100 (high+RFP)
| 288.1
| 1.90
| 2.33
| D-8
| No
|
BBa_J61002-BBa_J23106 (med.+RFP)
| 162.2
| 1.93
| 2.31
| D-9
| No
|
BBa_J61002-BBa_J23109 (low+RFP)
| 114.2
| 1.93
| 2.28
| D-10
| No
|
Transporters
HmtA
After 2 weeks of PCR-ing plasmid isolations there is a product worth wile to send for sequencing. 4,5,6, and 8
In the first 4 lanes PCR2 (261bp) is created from HmtA-AC3 plasmid with PstI 's left in place. In lane 5 till 8 PCR1 (1153bp)is made from HmtA-AC3 plasmid with PstI 's left in place to make mega primers to mutate the restriction sites.
In the lower left quarter is a PCR on HmtA-AC3 plasmid with PstI 's left in place with VF2 VR and failed.
The second lower quarter is a XbaI restriction control on plasmids 4,5,6 and 8 not completely digested but indicating the construct is correct in the way that there is a restriction site of the bio brick, meaning the EcoRI mutation got removed.
Metal Accumulation
- ligate MymT in pSB1AC3
- Purify mymT-RBS-pre/suff from gel using Roche Gel Extraction kit
- Elute in 25uL TE buffer (pH8)
- Digest mymT-RBS-pre/suff with EcoRI and Spe I
- Ligate with pSB1AC3/EcoRI-SpeI
Vectors
Dry
Jasper changed the model to take dimeric binding of ArsR into account and to ignore ArsD, as well as putting an image map on-line as the first step towards a clearer interface. The new model leads to a number of third order equations, which means that it is no longer feasible to give closed-form solutions, but it does seem perfectly feasible to compute the solutions numerically (as demonstrated by the updated calculator on the accumulation page).
April
|
M | T | W | T | F | S | S
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|
May
|
M | T | W | T | F | S | S
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_May_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_May_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
|
|
June
|
M | T | W | T | F | S | S
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_June_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
|
[http://2009.igem.org/Team:Groningen/Notebook/8_June_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
|
[http://2009.igem.org/Team:Groningen/Notebook/15_June_2009 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_June_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_June_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_June_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_June_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_June_2009 21]
|
[http://2009.igem.org/Team:Groningen/Notebook/22_June_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_June_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_June_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_June_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|
July
|
M | T | W | T | F | S | S
|
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_July_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_July_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
|
[http://2009.igem.org/Team:Groningen/Notebook/6_July_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_July_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_July_2009 8]
| [http://2009.igem.org/Team:Groningen/Notebook/9_July_2009 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
|
[http://2009.igem.org/Team:Groningen/Notebook/13_July_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_July_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_July_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
|
[http://2009.igem.org/Team:Groningen/Notebook/20_July_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_July_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_July_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_July_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_July_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_July_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
|
[http://2009.igem.org/Team:Groningen/Notebook/27_July_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_July_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_July_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_July_2009 30]
| [http://2009.igem.org/Team:Groningen/Notebook/31_July_2009 31]
|
|
August
|
M | T | W | T | F | S | S
|
|
|
|
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_August_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
|
[http://2009.igem.org/Team:Groningen/Notebook/3_August_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_August_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_August_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_August_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_August_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_August_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
|
[http://2009.igem.org/Team:Groningen/Notebook/10_August_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_August_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_August_2009 12]
| [http://2009.igem.org/Team:Groningen/Notebook/13_August_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_August_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_August_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_August_2009 16]
|
[http://2009.igem.org/Team:Groningen/Notebook/17_August_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_August_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_August_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_August_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_August_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_August_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_August_2009 23]
|
[http://2009.igem.org/Team:Groningen/Notebook/24_August_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_August_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_August_2009 26]
| [http://2009.igem.org/Team:Groningen/Notebook/27_August_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_August_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_August_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_August_2009 30]
|
[http://2009.igem.org/Team:Groningen/Notebook/31_August_2009 31]
|
|
September
|
M | T | W | T | F | S | S
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_September_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_September_2009 2]
| [http://2009.igem.org/Team:Groningen/Notebook/3_September_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_September_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_September_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_September_2009 6]
|
[http://2009.igem.org/Team:Groningen/Notebook/7_September_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_September_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_September_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_September_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_September_2009 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
|
[http://2009.igem.org/Team:Groningen/Notebook/14_September_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_September_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_September_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_September_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_September_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_September_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
|
[http://2009.igem.org/Team:Groningen/Notebook/21_September_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_September_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_September_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_September_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
|
[http://2009.igem.org/Team:Groningen/Notebook/28_September_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_September_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_September_2009 30]
|
|
October
|
M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_October_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
|
[http://2009.igem.org/Team:Groningen/Notebook/5_October_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_October_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
|
[http://2009.igem.org/Team:Groningen/Notebook/19_October_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
|
|
November
|
M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_November_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_November_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|