Team:UQ-Australia/Notebook

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<a href="https://2009.igem.org/Team:UQ-Australia/Lab" style="color: white">Our Lab
<a href="https://2009.igem.org/Team:UQ-Australia/Lab" style="color: white">Our Lab
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='''Notebook'''=
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{| align="center"
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|-valign="top"
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|{{#calendar: title=UQ-Australia |year=2009 | month=07}}
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|{{#calendar: title=UQ-Australia |year=2009 | month=08}}
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|{{#calendar: title=UQ-Australia |year=2009 | month=09}}
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|{{#calendar: title=UQ-Australia |year=2009 | month=10}}
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|}
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=='''Water Purification Project'''==
 
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'''24/07'''<br/>
 
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Mini prep DNA extractions performed for MS427-pBAD, MS427-pKKJ143 cultures incubated overnight.<br/>
 
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--> run on ethidium bromide gel to confirm DNA production.<br/>
 
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<br/>
 
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'''23/07/09''' <br/>
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===UQ-Australia is involved in two projects:===
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''E. coli'' incubated overnight in duplicate under the following conditions:<br/>
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Strain: MS427 <br/>
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Plasmid: pBAD (empty plasmid -AG43) <br/>
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- 2mL LB broth <br/>
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- 2µL ampicillin <br/>
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Strain: MS427 <br/>
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Plasmid: pKKJ143 (plasmid w/ AG43) <br/>
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- 2mL LB broth <br/>
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- 2µL ampicillin<br/>
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'''17/07/09  - MG1655 Stock Preparation''' <br/>
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* [https://2009.igem.org/Team:UQ-Australia/Notebook/Project1 '''Bioaccumulation (Mercury) Project''']
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MG1655 ''E. coli'' grown on pure LB stock. No growth was observed using LB + ampicillin medium.<br/>
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* [https://2009.igem.org/Team:UQ-Australia/Notebook/Project2 '''Bioprecipitation Project''']
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Pelleted and resuspended in 50:50 glycerol:LB medium and stored at -80 degrees C.<br/>
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Please click on the links above to check out each Lab Notebook.
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Bon apetite!
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=='''Bioprecipitation Project'''==
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'''5/08/09'''
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Plasmids have arrived!
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*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-E/J pXCK-E/J]
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*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-K pXCK-K]
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*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-EL pXCK-EL]
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*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-ES pXCK-ES]
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We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution.
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A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight.
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''Click on the plasmid name to look at the vector''
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'''6/08/09'''
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Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids.
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LB media was poured on petri dishes and plasmid DNA was checked again using Nanodrop.
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*pXCK-EL = 10.55 ng/uL
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*pXCK-ES = 8.43 ng/uL
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*pXCK-K = 11.73 ng/uL
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*pXCK-E/J = 2.66 ng/uL
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Plasmids were stored and transformation will be done on Monday.
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'''7/08/09'''
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Vectors were examined and we are planning for the best cloning strategy for the standard plasmids.
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'''10/08/09'''
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Transformation of our 4 plasmids took place. They were transformed into TOP10 bacteria. A colony check will be preformed tomorrow morning.
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To see protocol, click [https://2009.igem.org/Team:UQ-Australia/Notebook/Transformation_procedure HERE]
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'''11/08/09'''
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No results were given from the transformation. Transformation will have to be repeated.
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Latest revision as of 13:05, 21 October 2009

Notebook

July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_July_2009&action=edit 1] [http://2009.igem.org/UQ-Australia/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/14_July_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_July_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_July_2009&action=edit 16] [http://2009.igem.org/UQ-Australia/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_July_2009&action=edit 20] [http://2009.igem.org/UQ-Australia/21_July_2009 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_July_2009&action=edit 22] [http://2009.igem.org/UQ-Australia/23_July_2009 23] [http://2009.igem.org/UQ-Australia/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_July_2009&action=edit 26]
[http://2009.igem.org/UQ-Australia/27_July_2009 27] [http://2009.igem.org/UQ-Australia/28_July_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/2_August_2009&action=edit 2]
[http://2009.igem.org/UQ-Australia/3_August_2009 3] [http://2009.igem.org/UQ-Australia/4_August_2009 4] [http://2009.igem.org/UQ-Australia/5_August_2009 5] [http://2009.igem.org/UQ-Australia/6_August_2009 6] [http://2009.igem.org/UQ-Australia/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_August_2009&action=edit 9]
[http://2009.igem.org/UQ-Australia/10_August_2009 10] [http://2009.igem.org/UQ-Australia/11_August_2009 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_August_2009&action=edit 12] [http://2009.igem.org/UQ-Australia/13_August_2009 13] [http://2009.igem.org/UQ-Australia/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_August_2009&action=edit 16]
[http://2009.igem.org/UQ-Australia/17_August_2009 17] [http://2009.igem.org/UQ-Australia/18_August_2009 18] [http://2009.igem.org/UQ-Australia/19_August_2009 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_August_2009&action=edit 23]
[http://2009.igem.org/UQ-Australia/24_August_2009 24] [http://2009.igem.org/UQ-Australia/25_August_2009 25] [http://2009.igem.org/UQ-Australia/26_August_2009 26] [http://2009.igem.org/UQ-Australia/27_August_2009 27] [http://2009.igem.org/UQ-Australia/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_August_2009&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/UQ-Australia/1_September_2009 1] [http://2009.igem.org/UQ-Australia/2_September_2009 2] [http://2009.igem.org/UQ-Australia/3_September_2009 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_September_2009&action=edit 6]
[http://2009.igem.org/UQ-Australia/7_September_2009 7] [http://2009.igem.org/UQ-Australia/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_September_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_September_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_September_2009&action=edit 13]
[http://2009.igem.org/UQ-Australia/14_September_2009 14] [http://2009.igem.org/UQ-Australia/15_September_2009 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_September_2009&action=edit 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_October_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/2_October_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_October_2009&action=edit 4]
[http://2009.igem.org/UQ-Australia/5_October_2009 5] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_October_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/7_October_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_October_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_October_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_October_2009&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_October_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_October_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/14_October_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_October_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_October_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/17_October_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_October_2009&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_October_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_October_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_October_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_October_2009&action=edit 31]

UQ-Australia is involved in two projects:

Please click on the links above to check out each Lab Notebook.

Bon apetite!

Retrieved from "http://2009.igem.org/Team:UQ-Australia/Notebook"