Team:Cambridge/Research
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=Research= | =Research= | ||
- | === | + | ===Modelling=== |
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+ | http://www.wired.com/wiredscience/2009/08/zombies/ | ||
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+ | === Sensitivity Tuner === | ||
====Response of the pBAD promoter to arabinose concentration==== | ====Response of the pBAD promoter to arabinose concentration==== | ||
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The original paper, where they first isolated the operon: http://www.springerlink.com/content/q012rv3061829185/fulltext.pdf | The original paper, where they first isolated the operon: http://www.springerlink.com/content/q012rv3061829185/fulltext.pdf | ||
+ | |||
+ | Characterisation of the Violcein pigment: http://www.springerlink.com/content/k007400617283317/fulltext.pdf | ||
====Pseudomonas==== | ====Pseudomonas==== | ||
- | Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO: | + | Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO: http://jb.asm.org/cgi/content/full/183/21/6454 |
+ | |||
+ | ====Melanin==== | ||
+ | Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli: http://aem.asm.org/cgi/reprint/74/4/1190 | ||
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Latest revision as of 18:44, 21 October 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
-
Protocols
Stock List
Research
Shared Links and Help
Research
Modelling
http://www.wired.com/wiredscience/2009/08/zombies/
Sensitivity Tuner
Response of the pBAD promoter to arabinose concentration
[http://www.ncbi.nlm.nih.gov/pubmed/7608087 Tight regulation, modulation and and high level expression by vectors containing the arabinose pBAD promoter, Guzman et al. 1995]
[http://www3.interscience.wiley.com/journal/121400517/abstract?CRETRY=1&SRETRY=0 A high throughput approach to promoter study using Green Fluorescent, Pigment Rao et al. 2008]
AraC changes conformation in the presence of arabinose to permit transcription. It is always bound in the region around pBAD:
[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCY-41S4XRM-G&_user=1495569&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=b367a1ca739f73db8c72c07a3ec217f0 Regulation of the L-Arabinose Operon in Escherichia Coli, Schleif 2000]
Pigments
Violacein
Most recent paper: Information about new colours and VioE: http://www3.interscience.wiley.com/cgi-bin/fulltext/112732008/PDFSTART
Older paper with first characterisation of the vio operon: http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf
The original paper, where they first isolated the operon: http://www.springerlink.com/content/q012rv3061829185/fulltext.pdf
Characterisation of the Violcein pigment: http://www.springerlink.com/content/k007400617283317/fulltext.pdf
Pseudomonas
Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO: http://jb.asm.org/cgi/content/full/183/21/6454
Melanin
Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli: http://aem.asm.org/cgi/reprint/74/4/1190