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Uppsala-Sweden/27 August 2009
From 2009.igem.org
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--[[User:Anders|Anders]] 17:28, 27 August 2009 (UTC) | --[[User:Anders|Anders]] 17:28, 27 August 2009 (UTC) | ||
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| + | ==Competent cells, making (again!!)== | ||
| + | As our competent cells were again crap (!!) we just have to bite the bullet and make new ones. This time we use a vial of Invitrogen Top10 cells as seed culture. | ||
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| + | We also had to make new SOB buffer (as made previoulsy) and in the process made the discovery that our old SOB buffer wasn't pH corrected, it was at pH 6,3 instead of the recommended 7,5! Might very well be the couse of our failed competent cells. | ||
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| + | --[[User:Anders|Anders]] 10:41, 28 August 2009 (UTC) | ||
Latest revision as of 10:41, 28 August 2009
Assembly evaluation
Here are he images of the evaluation gel f yesterdays assembly:
Upper
Lower
To be annotated.
--Anders 17:28, 27 August 2009 (UTC)
Competent cells, making (again!!)
As our competent cells were again crap (!!) we just have to bite the bullet and make new ones. This time we use a vial of Invitrogen Top10 cells as seed culture.
We also had to make new SOB buffer (as made previoulsy) and in the process made the discovery that our old SOB buffer wasn't pH corrected, it was at pH 6,3 instead of the recommended 7,5! Might very well be the couse of our failed competent cells.
--Anders 10:41, 28 August 2009 (UTC)
