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Virginia Commonwealth/6 July 2009
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==Monday 06 July 2009== | ==Monday 06 July 2009== | ||
===Results=== | ===Results=== | ||
| - | * | + | * n/a |
| - | + | ||
---- | ---- | ||
| + | |||
===Tasks=== | ===Tasks=== | ||
| + | ''Maria and Afton'' | ||
| + | *Gather information and choose parts for experiments (promoter, backbone, and RET) | ||
| + | *Write running draft | ||
| + | *Transfer information to new lab notebook | ||
| + | [[User:Trentay|Trentay]] 19:36, 6 July 2009 (UTC) | ||
| + | |||
| + | |||
| + | ''Kevin and Adam'' | ||
| + | *Continue research and further analyze and hone the list of DNA sequences to be synthesized | ||
| + | |||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa_I742111, BBa_K118024, and BBa_K118025). BBa_I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa_K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa_K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive. | ||
---- | ---- | ||
| - | |||
| - | |||
====Wetlab==== | ====Wetlab==== | ||
| - | + | ''Maria and Afton'' | |
| - | * | + | *n/a |
| + | [[User:Trentay|Trentay]] 19:36, 6 July 2009 (UTC) | ||
Latest revision as of 15:45, 10 August 2009
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Contents |
Monday 06 July 2009
Results
- n/a
Tasks
Maria and Afton
- Gather information and choose parts for experiments (promoter, backbone, and RET)
- Write running draft
- Transfer information to new lab notebook
Trentay 19:36, 6 July 2009 (UTC)
Kevin and Adam
- Continue research and further analyze and hone the list of DNA sequences to be synthesized
Craig and Clay
- It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa_I742111, BBa_K118024, and BBa_K118025). BBa_I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa_K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa_K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive.
Wetlab
Maria and Afton
- n/a
Trentay 19:36, 6 July 2009 (UTC)
