"
Uppsala-Sweden/31 August 2009
From 2009.igem.org
Karl.brune (Talk | contribs) |
|||
| (3 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
==Adh2Y PCR== | ==Adh2Y PCR== | ||
| - | + | [[Image:20090831_Adh2Y-labelled.jpg]] | |
| - | [[Image: | + | |
| - | + | ==Colony PCR of Transformations (RBS+pdc+RBS+adh2z (RPRB), pPetE+RBS, RBS-pirB-Ter== | |
| - | --[[User: | + | |
| + | Colony PCR of Transformations (RBS+pdc+RBS+adh2z (RPRB), pPetE+RBS, RBS-pirB-Ter with VF2 and VR primers in 10ml reactions. 50°C annealing for 45 sec, 72° elongation for 3 min, 40 cylces | ||
| + | |||
| + | With self ligated vector (pSB1A3) as control | ||
| + | |||
| + | ==Gel of Colony PCR== | ||
| + | |||
| + | [[Image:20090831_colonypcr-labelled.jpg]] | ||
| + | |||
| + | [[Image:20090831_colonypcr_p2-labelled.jpg]] | ||
| + | |||
| + | Gel of Adh2Y PCR with BBa extension primers on Bakers Yeast lysate (Wine yeast did not work). | ||
| + | --[[User:Karl.brune|Karl.brune]] 15:18, 1 September 2009 (UTC) | ||
==Transformation== | ==Transformation== | ||
| Line 16: | Line 28: | ||
--[[User:Anders|Anders]] 15:11, 31 August 2009 (UTC) | --[[User:Anders|Anders]] 15:11, 31 August 2009 (UTC) | ||
| + | |||
| + | Amounts to be used for Assembly: [[Media:Assembly volume calcuator090831_1.xls]] and Biobricking: [[Media:Ligation voulume calcuator_090831_1.xls]] | ||
| + | |||
| + | --[[User:Anders|Anders]] 16:11, 31 August 2009 (UTC) | ||
Latest revision as of 15:59, 1 September 2009
Contents |
Adh2Y PCR
Colony PCR of Transformations (RBS+pdc+RBS+adh2z (RPRB), pPetE+RBS, RBS-pirB-Ter
Colony PCR of Transformations (RBS+pdc+RBS+adh2z (RPRB), pPetE+RBS, RBS-pirB-Ter with VF2 and VR primers in 10ml reactions. 50°C annealing for 45 sec, 72° elongation for 3 min, 40 cylces
With self ligated vector (pSB1A3) as control
Gel of Colony PCR
File:20090831 colonypcr-labelled.jpg
File:20090831 colonypcr p2-labelled.jpg
Gel of Adh2Y PCR with BBa extension primers on Bakers Yeast lysate (Wine yeast did not work). --Karl.brune 15:18, 1 September 2009 (UTC)
Transformation
Digestion of RBS and Adh2Y PCR product prior to ligation. Media:Digestion(excert)090831.xls
--Anders 13:20, 31 August 2009 (UTC)
Digestion and cleanup report, 100 times dilution: Media:digest_purification_measur_09831.txt
--Anders 15:11, 31 August 2009 (UTC)
Amounts to be used for Assembly: Media:Assembly volume calcuator090831_1.xls and Biobricking: Media:Ligation voulume calcuator_090831_1.xls
--Anders 16:11, 31 August 2009 (UTC)
