Template:Team:KULeuven/2 September 2009/VanillinProduction

From 2009.igem.org

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* The 4 grown colonies are put on liquid culture
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* The four grown colonies are put on liquid culture
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* New (again...) restriction digest on Sam5, Sam8, Fcs and Ech, genes were mini prepped on tuesday.
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* New (again...) restriction digest on ''sam5, sam8, fcs'' and ''ech'', genes were miniprepped on Tuesday.
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** Overnight in 16C
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** Overnight at 16°C
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* the plates with sam5, sam8, ech and fcs were re-ented. from sam5 and fcs for colonies were picked and plated out.
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* The plates with ''sam5, sam8, ech'' and ''fcs'' were re-ented. From ''sam5'' and ''fcs'' four colonies were picked and plated.
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* The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today.

Latest revision as of 11:57, 14 September 2009

  • The four grown colonies are put on liquid culture
  • New (again...) restriction digest on sam5, sam8, fcs and ech, genes were miniprepped on Tuesday.
    • Overnight at 16°C
  • The plates with sam5, sam8, ech and fcs were re-ented. From sam5 and fcs four colonies were picked and plated.
  • The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today.
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