Team:EPF-Lausanne

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==Concept==
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<html><center><a href="https://2009.igem.org/Team:EPF-Lausanne/Project_Abstract"><img src="https://static.igem.org/mediawiki/2009/4/44/Mainpage.jpg"></a></center></html>
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[[Image:LovTAP_dimer.png|right|300px|thumb|LovTAP dimer bound to DNA]]
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Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
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==Project Abstract==
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Recent discoveries of photoreceptors in many organisms gave us insights into a possible interest of using light responsive genetic tools in synthetic biology.  
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The final goal of our project is to induce a change in gene expression, more specifically to turn a gene on or off, in a living organism, in response to a light stimulus.
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We will use light sensitive DNA binding proteins, or light sensitive proteins that activate DNA binding proteins to transduce a light input into a chosen output, for example reporter genes like GFP, RFP.
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The genetic circuits allowing us to measure the activity and responsiveness of light sensitive proteins are already designed, whereas the parts and biobricks required to engineer these circuits are still in formation.
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If we demonstrate that the light-induced-gene switch tool works in vivo, it would show that easier and faster tools could be used in several fields of biology. It would induce more localized, more precise (time resolution) and drastically faster genetic changes than the current used tools, which will then allow research to evolve even better.
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For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.
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LOV means light, oxygen, and voltage, whereas TAP means tryptophan-activated protein.
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The results clearly show that this light-induced gene switch tool works ''in vivo'', demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.
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Latest revision as of 22:45, 21 October 2009



Mainpage.jpg



Concept


LovTAP dimer bound to DNA

Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.

For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.

The results clearly show that this light-induced gene switch tool works in vivo, demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.





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