Team:Warsaw/Calendar-Main/3 September 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> ===<div style="text-align: center;">Acquiring the internaline A gene from ''Listeria monocytogenes'' st. EDG-e genome </div>=== '''Jarek'''...)
 
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===<div style="text-align: center;"><h3>Preparation of glycerol stocks</h3></div>===
 +
'''<h4>Monika</h4>'''
 +
 +
 +
*Preparation of glycerol stocks following the prodcedure described [https://2009.igem.org/Team:Warsaw/protocols#Preparation_of_glycerol_stocks <span style="color: blue;">here</span>]
 +
 +
 +
pAraC + GFP
 +
 +
plac + RBS + cI + ter
 +
 +
plac + RBS + cI + RBS + GFP + ter
 +
 +
J07037
 +
 +
plac
 +
 +
pSB6A1
 +
 +
 +
<h3><div style="text-align: center;">Assembly of endosomal detection operon</div></h3>
 +
<h4>Marcin</h4>
 +
 +
Task 1:
 +
* Isolate the plasmids containing  following construct:
 +
* [http://partsregistry.org/Part:BBa_K177035<span style="color: black">BBa_K177035</span>] on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>] plasmid
 +
Methods:
 +
Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here]
 +
 +
Task 2:
 +
* Digestion of plasmids previously described constructs
 +
Methods:
 +
* Reaction mixture composition:
 +
<pre>
 +
20 μl purified plasmid DNA product
 +
1 μl XbaI (Fermentas)
 +
1 μl PstI (Fermentas)
 +
2 μl Buffer Tango (Fermentas)
 +
15 μl MQ water
 +
</pre>
 +
* Digest was performed about six hours and subsequently thermal inactivated
 +
 +
Task 3:
 +
* Gel-outs of construct described in Task 2
 +
Methods:
 +
* All gel-outs were performed using the EurX gel-out kit according to the manual
 +
 +
Task 4:
 +
 
 +
*  Ligation of following constructs:
 +
** [http://partsregistry.org/Part:BBa_K177037<span style="color: black">BBa_K177037</span>] on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>] plasmid
 +
** cro CDS on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>]
 +
** Bax CDS on [http://partsregistry.org/Part:pSB1A3<span style="color: black">pSB1A3</span>]
 +
Methods:
 +
* Reaction mixture composition:
 +
<pre>
 +
10 μl digested insert
 +
8 μl digested vector
 +
2 μl Tango buffer(Fermentas)
 +
3 μl dNTPs mixture (EurX, concentration 5 mM)
 +
1 μl ligase T4 (Fermentas)
 +
</pre>
 +
* Reaction were carried out 12 hours and subsequently thermally inactivated.
 +
 +
<html>
 +
<h3><div style="text-align: center;">Cloning of the mgtc promoter into the pSB1A3 plasmid</div></h3>
 +
<h4>Kamil</h4>
 +
<br />
 +
<p>Tasks:</p>
 +
<ul>
 +
<li>Plasmid isolation</li>
 +
<li>Control digest</li>
 +
</ul>
 +
<br />
 +
<p>Methods:</p>
 +
<ul>
 +
<li>Plasmids from 3 ml of overnight culture were isolated using the Plasmid Mini kit (A&A Biotechnology).</li>
 +
<li>The digest mix was prepared as follows:
 +
<p><pre>20&mu;l isolated plasmid<br/>3&mu;l Buffer O (Fermentas)<br/>1&mu;l PstI enzyme<br/>1&mu;l EcoRI enzyme<br/>5&mu;l H<sub>2</sub>O</pre></p></li>
 +
<li>The digest was carried out for 3h in 37&deg;C and then inactivated in 80&deg;C for 15min.</li>
 +
<li>The results were visualised on 1% agarose gel</li>
 +
</ul>
 +
<br />
 +
<p>Results:</p>
 +
<ul>
 +
<img src="https://static.igem.org/mediawiki/2009/6/65/2009.09.03.jpg">
 +
<li>First four bands (besides the the size ruler) show empty plasmids.</li>
 +
</ul>
 +
<br/>
 +
<p>Conclusions:</p>
 +
<ul>
 +
<li>Respawn and try again in... 3 days.</li>
 +
</ul>
 +
<br/>
 +
 +
 +
<h3><div style="text-align: center;">Cloning of the cro-box into the pSB1A3 plasmid</div></h3>
 +
<h4>Kamil</h4>
 +
<br />
 +
<p>Tasks:</p>
 +
<ul>
 +
<li>Plasmid isolation</li>
 +
<li>Control digest</li>
 +
</ul>
 +
<br />
 +
<p>Methods:</p>
 +
<ul>
 +
<li>Plasmids from 3 ml of overnight culture were isolated using the Plasmid Mini kit (A&A Biotechnology).</li>
 +
<li>The digest mix was prepared as follows:
 +
<p><pre>20&mu;l isolated plasmid<br/>3&mu;l Buffer O (Fermentas)<br/>1&mu;l PstI enzyme<br/>1&mu;l EcoRI enzyme<br/>5&mu;l H<sub>2</sub>O</pre></p></li>
 +
<li>The digest was carried out for 3h in 37&deg;C and then inactivated in 80&deg;C for 15min.</li>
 +
<li>The results were visualised on 1% agarose gel</li>
 +
</ul>
 +
<br />
 +
<p>Results:</p>
 +
<ul>
 +
<img src="https://static.igem.org/mediawiki/2009/6/65/2009.09.03.jpg">
 +
<li>Final five bands show empty plasmids.</li>
 +
</ul>
 +
<br/>
 +
<p>Conclusions:</p>
 +
<ul>
 +
<li>Respawn and try again in... 3 days.</li>
 +
</ul>
 +
<br/>
 +
<h3><div style="text-align: center;">Cotransformation to obtain cells with following plasmid combinations [<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>,<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>][<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>,<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177038">K177038</a>][<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>,<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>][<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>,<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177038">K177038</a>]
 +
<h4>Ania</h4>
 +
<br/>
 +
<p>Tasks:</p>
 +
<ul>
 +
<li>
 +
</li>
 +
</ul>
 +
<br/>
 +
</html>

Latest revision as of 22:16, 20 September 2009


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Contents

Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome

Jarek

Tasks:

  • Digestion of PCR products, separetly with MboI and CaiI restriction endonucleases
  • Electrophoretical segregation of digested samples in 1,5% agarose gel
  • Preparation of another PCR reaction with L.monocytogenes genome DNA


Results:

  • There were no visible products of digestion, even after long treatment with ethidium bromide. Propably the Clean-Up kit isn't working well. I'll try to acquire IntA gene with another PCR.


Preparation of glycerol stocks

Monika


  • Preparation of glycerol stocks following the prodcedure described here


pAraC + GFP

plac + RBS + cI + ter

plac + RBS + cI + RBS + GFP + ter

J07037

plac

pSB6A1


Assembly of endosomal detection operon

Marcin

Task 1:

  • Isolate the plasmids containing following construct:
  • [http://partsregistry.org/Part:BBa_K177035BBa_K177035] on [http://partsregistry.org/Part:pSB1A3pSB1A3] plasmid

Methods: Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here]

Task 2:

  • Digestion of plasmids previously described constructs

Methods:

  • Reaction mixture composition: 
20 μl purified plasmid DNA product
1 μl XbaI (Fermentas)
1 μl PstI (Fermentas) 
2 μl Buffer Tango (Fermentas)
15 μl MQ water
  • Digest was performed about six hours and subsequently thermal inactivated

Task 3:

  • Gel-outs of construct described in Task 2

Methods:

  • All gel-outs were performed using the EurX gel-out kit according to the manual

Task 4:

  • Ligation of following constructs:
    • [http://partsregistry.org/Part:BBa_K177037BBa_K177037] on [http://partsregistry.org/Part:pSB1A3pSB1A3] plasmid
    • cro CDS on [http://partsregistry.org/Part:pSB1A3pSB1A3]
    • Bax CDS on [http://partsregistry.org/Part:pSB1A3pSB1A3]

Methods:

  • Reaction mixture composition: 
10 μl digested insert
8 μl digested vector 
2 μl Tango buffer(Fermentas)
3 μl dNTPs mixture (EurX, concentration 5 mM) 
1 μl ligase T4 (Fermentas)
  • Reaction were carried out 12 hours and subsequently thermally inactivated.

Cloning of the mgtc promoter into the pSB1A3 plasmid

Kamil


Tasks:

  • Plasmid isolation
  • Control digest

Methods:

  • Plasmids from 3 ml of overnight culture were isolated using the Plasmid Mini kit (A&A Biotechnology).
  • The digest mix was prepared as follows: 

    20μl isolated plasmid
    3μl Buffer O (Fermentas)
    1μl PstI enzyme
    1μl EcoRI enzyme
    5μl H2O

  • The digest was carried out for 3h in 37°C and then inactivated in 80°C for 15min.
  • The results were visualised on 1% agarose gel

Results:

  • First four bands (besides the the size ruler) show empty plasmids.

Conclusions:

  • Respawn and try again in... 3 days.

Cloning of the cro-box into the pSB1A3 plasmid

Kamil


Tasks:

  • Plasmid isolation
  • Control digest

Methods:

  • Plasmids from 3 ml of overnight culture were isolated using the Plasmid Mini kit (A&A Biotechnology).
  • The digest mix was prepared as follows: 

    20μl isolated plasmid
    3μl Buffer O (Fermentas)
    1μl PstI enzyme
    1μl EcoRI enzyme
    5μl H2O

  • The digest was carried out for 3h in 37°C and then inactivated in 80°C for 15min.
  • The results were visualised on 1% agarose gel

Results:

  • Final five bands show empty plasmids.

Conclusions:

  • Respawn and try again in... 3 days.

Cotransformation to obtain cells with following plasmid combinations [K177012,K177011][K177012,K177038][K177033,K177011][K177033,K177038]

Ania


Tasks:




April
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_April_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_April_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_April_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_April_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_April_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_April_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_April_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_April_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_April_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_April_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_April_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_April_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_April_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_April_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_April_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_April_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_April_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_April_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_April_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_April_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_April_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_April_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_April_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_April_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_April_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_April_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_April_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_April_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_April_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_April_2009 30]
May
MTWTFSS
        [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_May_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_May_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_May_2009 3]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/4_May_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_May_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_May_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_May_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_May_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_May_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_May_2009 10]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/11_May_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_May_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_May_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_May_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_May_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_May_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_May_2009 17]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/18_May_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_May_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_May_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_May_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_May_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_May_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_May_2009 24]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/25_May_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_May_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_May_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_May_2009 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_June_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_June_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_June_2009 28]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/29_June_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_July_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_July_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_July_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_July_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_July_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_July_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_July_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_July_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_July_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_July_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_July_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_July_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_July_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_July_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_July_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_July_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_July_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_July_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_July_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_July_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_July_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_July_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_July_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_July_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_July_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_July_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_July_2009 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_July_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_August_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_August_2009 2]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/3_August_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_August_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_August_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_August_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_August_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_August_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_August_2009 9]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/10_August_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_August_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_August_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_August_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_August_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_August_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_August_2009 16]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/17_August_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_August_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_August_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_August_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_August_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_August_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_August_2009 23]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/24_August_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_August_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_August_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_August_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_August_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_August_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_August_2009 30]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_September_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_September_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_September_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_September_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_September_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_September_2009 6]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/7_September_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_September_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_September_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_September_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_September_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_September_2009 13]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/14_September_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_September_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_September_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_September_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_September_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_September_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_September_2009 20]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/21_September_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_September_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_September_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_September_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_September_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_September_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_September_2009 27]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/28_September_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_September_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_October_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_October_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_October_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_October_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_October_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/28_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
Retrieved from "http://2009.igem.org/Team:Warsaw/Calendar-Main/3_September_2009"