Team:Warsaw/Calendar-Main/24 August 2009
From 2009.igem.org
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Making of the plac-RBS-llo-intA part</div></h3> <h4>Jarek</h4> <br /> <p>Tasks:</p> <ul> <li>Pre...) |
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<li>Preparation of 24 liquid cultures from colonies that grew after transformation in LB+Amp.</li> | <li>Preparation of 24 liquid cultures from colonies that grew after transformation in LB+Amp.</li> | ||
<li>Incubation of liquid cultures in 37C degree.</li> | <li>Incubation of liquid cultures in 37C degree.</li> | ||
+ | <li>Isolation of plasmid DNA from liquid cultures.</li> | ||
+ | <li>Digestion of the isolated DNA with EcoRI endonuclease.</li> | ||
</ul> | </ul> | ||
<br /> | <br /> | ||
+ | <h3><div style="text-align: center;">Cloning switch 1 regulatory parts [ | ||
+ | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>-PcI.RBS.LacI, | ||
+ | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, | ||
+ | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>-PLacI.RBS.cI.terminator, | ||
+ | <a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator | ||
+ | ] | ||
+ | into two compatible low copy number plasmids of different antibiotic resistance</div></h3> | ||
+ | <h4>Ania</h4> | ||
+ | <br/> | ||
+ | <p>Tasks:</p> | ||
+ | <ul> | ||
+ | <li> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <br/> | ||
</html> | </html> | ||
Latest revision as of 00:15, 10 September 2009
Making of the plac-RBS-llo-intA part
Jarek
Tasks:
- Preparation of 24 liquid cultures from colonies that grew after transformation in LB+Amp.
- Incubation of liquid cultures in 37C degree.
- Isolation of plasmid DNA from liquid cultures.
- Digestion of the isolated DNA with EcoRI endonuclease.
Ania
Tasks:
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