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EPF-Lausanne/3 September 2009
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==Wet Lab== | ==Wet Lab== | ||
* '''Results of the RO1:''' | * '''Results of the RO1:''' | ||
| - | + | There is clones on every plate! | |
| - | : → colony PCR on all clones with | + | : → colony PCR on all clones with the Taq Platinium protocol |
| - | + | <br> | |
* '''Results from the media try:''' | * '''Results from the media try:''' | ||
| - | + | All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture. | |
{| class="wikitable" style="text-align:center; width:80%;" | {| class="wikitable" style="text-align:center; width:80%;" | ||
| Line 50: | Line 50: | ||
|LB | |LB | ||
|- | |- | ||
| - | ! scope=row | | + | ! scope=row | M9/min + AA + thiamine |
|rose | |rose | ||
|white | |white | ||
|white | |white | ||
|- | |- | ||
| - | ! scope=row | | + | ! scope=row | M9/min + thiamine |
|rose | |rose | ||
|rose | |rose | ||
| Line 67: | Line 67: | ||
| + | * '''Culture for characterization''' | ||
| + | We tried the following media, with and without Trp : | ||
| + | : LB | ||
| + | : M9/min + thiamine | ||
| + | : M9/min + AA + glycerol | ||
| + | : M9/min + thiamine | ||
| - | + | The gel showed : | |
| + | : '''RO1''': it works fine, all our constructs have the TrpO | ||
| + | : '''RO2''': #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct! | ||
==People in the lab== | ==People in the lab== | ||
Latest revision as of 14:32, 8 September 2009
Contents |
Wet Lab
- Results of the RO1:
There is clones on every plate!
- → colony PCR on all clones with the Taq Platinium protocol
- Results from the media try:
All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
| RO2 #6 | RO2 #7 | RO2 #8 | |
|---|---|---|---|
| M9/min + AA + glycerol | white | white | white |
| LB | LB + slight red | LB + red | LB |
| M9/min + AA + thiamine | rose | white | white |
| M9/min + thiamine | rose | rose | white |
| SC | NA | NA | NA |
- Culture for characterization
We tried the following media, with and without Trp :
- LB
- M9/min + thiamine
- M9/min + AA + glycerol
- M9/min + thiamine
The gel showed :
- RO1: it works fine, all our constructs have the TrpO
- RO2: #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!
People in the lab
Basile, Mélanie, Caroline
