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Team:Imperial College London/Wetlab/Protocols/Cellculture
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| <b>CC1</b>: Miniprep | | <b>CC1</b>: Miniprep | ||
| [[Team:Imperial_College_London/ |Link]] | | [[Team:Imperial_College_London/ |Link]] | ||
| - | |* Harvest | + | | * Harvest smaller amounts of genomic DNA<br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC2</b>: Cellulase | | <b>CC2</b>: Cellulase | ||
| [[Team:Imperial_College_London/ |Link]] | | [[Team:Imperial_College_London/ |Link]] | ||
| - | |*Harvest | + | | * Harvest larger amount of genomic DNA<br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC3</b>: Cell competence | | <b>CC3</b>: Cell competence | ||
| [[Team:Imperial_College_London/ |Link]] | | [[Team:Imperial_College_London/ |Link]] | ||
| - | | *Making cells more competent to taking up DNA <br> | + | | * Making cells more competent to taking up DNA <br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC4</b>: Ligation | | <b>CC4</b>: Ligation | ||
| [[Team:Imperial_College_London/ |Link]] | | [[Team:Imperial_College_London/ |Link]] | ||
| - | | *Ligation of different DNA constructs together<<br> | + | | * Ligation of different DNA constructs together<<br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC5</b>: Transformation (BL21) | | <b>CC5</b>: Transformation (BL21) | ||
| [[Team:Imperial_College_London/ |Link]] | | [[Team:Imperial_College_London/ |Link]] | ||
| - | | *Transformation into BL21 cells using electrical shock <<br> | + | | * Transformation into BL21 cells using electrical shock <<br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC6</b>: Transformation (Top10) | | <b>CC6</b>: Transformation (Top10) | ||
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/TransTop10 |Transformation into Top10]] | | [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/TransTop10 |Transformation into Top10]] | ||
| - | | *Transformation into chemically competent Top10 cells <<br> | + | | * Transformation into chemically competent Top10 cells <<br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC7</b>: Make LB plates | | <b>CC7</b>: Make LB plates | ||
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]] | | [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]] | ||
| - | | *Making plates of LB agar for cell culturing<br> | + | | * Making plates of LB agar for cell culturing<br> |
| - | + | ||
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>CC7</b>: Extract DNA from registry | | <b>CC7</b>: Extract DNA from registry | ||
| [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]] | | [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]] | ||
| - | | *Extracting the required DNA from the registry<br> | + | | * Extracting the required DNA from the registry<br> |
| + | |||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | | <b>CC13</b>: Genome Prep for Restriction asay | ||
| + | | [[Team:Imperial_College_London/Wetlab/Protocols/GenomePrep |Genome Prep]] | ||
| + | | * Preperation of the genomic DNA for restriction digest assay. | ||
| + | |||
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|} | |} | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 11:05, 30 September 2009
Cell culture protocols
| Section | Assay | Overview and Aims |
|---|---|---|
| CC1: Miniprep | Link | * Harvest smaller amounts of genomic DNA |
| CC2: Cellulase | Link | * Harvest larger amount of genomic DNA |
| CC3: Cell competence | Link | * Making cells more competent to taking up DNA |
| CC4: Ligation | Link | * Ligation of different DNA constructs together< |
| CC5: Transformation (BL21) | Link | * Transformation into BL21 cells using electrical shock < |
| CC6: Transformation (Top10) | Transformation into Top10 | * Transformation into chemically competent Top10 cells < |
| CC7: Make LB plates | Make LB plates | * Making plates of LB agar for cell culturing |
| CC7: Extract DNA from registry | Extracting DNA | * Extracting the required DNA from the registry |
| CC13: Genome Prep for Restriction asay | Genome Prep | * Preperation of the genomic DNA for restriction digest assay.
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