Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation

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(Calibration protocols (CA))
(Calibration protocols (CA))
 
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=Calibration protocols (CA)=
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=Promoter Characterisation (PC)=
{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3"
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! Overview and Aims
! Overview and Aims
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| <b>2.Auto-Induction</b>
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| <b>Promoter Characterisation</b>
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
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Latest revision as of 10:56, 30 September 2009

Promoter Characterisation (PC)

Section Assay Overview and Aims
Promoter Characterisation IPTG
  • Characterise Lac promoter by varying concentrations of IPTG
  • Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

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