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Team:Virginia Commonwealth/Internal/Promoter characterization
From 2009.igem.org
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| - | * | + | *Let me know if this needs to go anywhere else. I will put them in order later. =) |
| + | [[User:MandM|MandM]] 23:39, 15 June 2009 (UTC) | ||
Latest revision as of 23:39, 15 June 2009
Promoter Characterization Project Planning
- Table of triplets to be composed together
| Promoter | Gene (includes RBS and Terminator) | Backbone | E. Coli type |
|---|---|---|---|
| J23100 | E0240 | pSB3K3 | NEB10β |
| J23101 | E0240 | pSB3K3 | NEB10β |
| J23102 | E0240 | pSB3K3 | NEB10β |
| J23103 | E0240 | pSB3K3 | NEB10β |
| J23104 | E0240 | pSB3K3 | NEB10β |
| J23105 | E0240 | pSB3K3 | NEB10β |
| J23107 | E0240 | pSB3K3 | NEB10β |
| J23110 | E0240 | pSB3K3 | NEB10β |
| J23113 | E0240 | pSB3K3 | NEB10β |
| J23116 | E0240 | pSB3K3 | NEB10β |
| R0011 | E0240 | pSB3K3 | NEB10β |
| R0040 | E0240 | pSB3K3 | NEB10β |
- Table of BioBrick parts, concentrations and amounts
| Part No. | Concentration (microgram/milliliter) | Amount (microgram) |
|---|---|---|
| J23100 | 14.0 | 5.32 |
| J23103 | 7.0 | 2.52 |
| J23105 | 8.0 | 2.96 |
| J23104 | 11.0 | 4.18 |
| J23101 | 12.0 | 4.56 |
| J23107 | 18.0 | 8.1 |
| J23116 | 20.0 | 7.2 |
| J23113 | 14.0 | 5.18 |
| J23110 | 12.0 | 4.2 |
| J23102 | 31.0 | 10.23 |
| R0011 | 21.0 | 5.46 |
| R0040 | 15.0 | 5.4 |
| pSB3K3 (Total) | n/a | 5.465 |
| pSB3K3 (1) | 24 | .984 |
| pSB3K3 (2) | 30 | 1.236 |
| pSB3K3 (3) | 31.5 | 1.310 |
| pSB3K3 (4) | 45 | 1.935 |
| E0240 (Total) | n/a | 6.574 |
| E0240 (1) | 30.0 | 1.200 |
| E0240 (2) | 37.5 | 1.575 |
| E0240 (3) | 45.0 | 1.868 |
| E0240 (4) | 49.5 | 1.931 |
- Let me know if this needs to go anywhere else. I will put them in order later. =)
MandM 23:39, 15 June 2009 (UTC)
