Team:PKU Beijing/Notebook/AND Gate 1/Input/LacI TetR
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{{PKU_Beijing/Sidebar_Notebook}} | {{PKU_Beijing/Sidebar_Notebook}} | ||
{{PKU_Beijing/Header2}} | {{PKU_Beijing/Header2}} | ||
+ | [[Team:PKU_Beijing/Notebook|Notebook]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1|AND Gate 1]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1/Input|Input]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1/Input/LacI_TetR|Molecular cloning: 6×RBS+ araC/ lacI]] | ||
==='''Molecular cloning: 6×RBS+ araC/ lacI'''=== | ==='''Molecular cloning: 6×RBS+ araC/ lacI'''=== | ||
[[Image:PKU_Shuke_Wu_LacI_TetR.JPG|400px]] | [[Image:PKU_Shuke_Wu_LacI_TetR.JPG|400px]] | ||
- | + | '''Resource:'''<br> | |
- | + | ||
6 RBS: from the parts: B0030, B0032, B0034, J61100, J61107, J61127; renamed as RBS1 RBS2…RBS6; <br> | 6 RBS: from the parts: B0030, B0032, B0034, J61100, J61107, J61127; renamed as RBS1 RBS2…RBS6; <br> | ||
araC: C0080<br> | araC: C0080<br> | ||
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lane 16~18: lacI+RBS6 1~3; | lane 16~18: lacI+RBS6 1~3; | ||
- | ''' | + | ==='''Result'''=== |
+ | |||
10 clones were successfully constructed: RBS1~5+lacI and tetR; <br> | 10 clones were successfully constructed: RBS1~5+lacI and tetR; <br> | ||
2 clones were failed: RBS6+lacI and tetR. | 2 clones were failed: RBS6+lacI and tetR. | ||
{{PKU_Beijing/Foot}} | {{PKU_Beijing/Foot}} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 18:34, 21 October 2009
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