Team:PKU Beijing/Notebook/AND Gate 1/Core/Siheng He
From 2009.igem.org
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{{PKU_Beijing/Sidebar_Notebook}} | {{PKU_Beijing/Sidebar_Notebook}} | ||
{{PKU_Beijing/Header2}} | {{PKU_Beijing/Header2}} | ||
+ | [[Team:PKU_Beijing/Notebook|Notebook]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1|AND Gate 1]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1/Core|Core]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1/Core/Siheng_He|Siheng He's Note]] | ||
==='''2009.9.4'''=== | ==='''2009.9.4'''=== | ||
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Miniprep S1, S2, S4<br> | Miniprep S1, S2, S4<br> | ||
Double digestion (EcoRI+PstI): S1, S2, S4<br> | Double digestion (EcoRI+PstI): S1, S2, S4<br> | ||
- | [[Image:PKU_20090904_Siheng_He_2.JPG|400px]] | + | <!--- [[Image:PKU_20090904_Siheng_He_2.JPG|400px]] ---> |
40 uL S1(sal-supD, A+K+) is digested using EcoRI and PstI<br> | 40 uL S1(sal-supD, A+K+) is digested using EcoRI and PstI<br> | ||
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'''21:00'''<br> | '''21:00'''<br> | ||
electrophoresis to identify the results of digestion<br> | electrophoresis to identify the results of digestion<br> | ||
- | [[Image:PKU_20090905_Siheng_He_2.JPG|400px]]<br> | + | <!--- [[Image:PKU_20090905_Siheng_He_2.JPG|400px]]<br> ---> |
Lane 1: plasmid<br> | Lane 1: plasmid<br> | ||
Lane 2: plasmid double digestion (EP)<br> | Lane 2: plasmid double digestion (EP)<br> | ||
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Recycle the Sal insert from gel. The insert is about 1.3 kb in length<br> | Recycle the Sal insert from gel. The insert is about 1.3 kb in length<br> | ||
- | [[Image:PKU_20090905_Siheng_He_3.JPG|400px]] | + | <!--- [[Image:PKU_20090905_Siheng_He_3.JPG|400px]] ---> |
DNA product purification: digested supD plasmid | DNA product purification: digested supD plasmid | ||
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'''14:00'''<br> | '''14:00'''<br> | ||
double digestion with EcoRI and PstI to identify: 5J 1~5, 2G 1~5 plasmid<br> | double digestion with EcoRI and PstI to identify: 5J 1~5, 2G 1~5 plasmid<br> | ||
- | [[Image:PKU_20090912_Siheng_He_1.JPG|400px]] | + | <!--- [[Image:PKU_20090912_Siheng_He_1.JPG|400px]] ---> |
==='''2009.9.22'''=== | ==='''2009.9.22'''=== |
Latest revision as of 19:05, 21 October 2009
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