Latest revision as of 18:02, 21 October 2009

Lab Book
Protocols

Meetings Calendar

Lab Book

Team Newcastle 2009 iGEM Animation22.gif

Introductory Lab Sessions (30th June - 27th July)

To train up the members of our team who hadn't experienced lab work before, and to sharpen the skills of the team members who had, our advisers planned a period of introductory lab sessions. These experiments were based around growing cell cultures and making E.coli cells competent whilst making us aware of important lab principles such as handling of equipment, aseptic technique, lab etiquette, etc. Please see calendar below:

June
M	T	W	T	F	S	S
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/1_June_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/2_June_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/3_June_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_June_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_June_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/6_June_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/8_June_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/9_June_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/10_June_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_June_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_June_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_June_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/15_June_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/16_June_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_June_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_June_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_June_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_June_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_June_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/23_June_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/24_June_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/25_June_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_June_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/27_June_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_June_2009&action=edit 29]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/30_June_2009 30]

July
M	T	W	T	F	S	S
		[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/1_July_2009 1]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/2_July_2009 2]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/3_July_2009 3]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_July_2009&action=edit 5]
[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/6_July_2009 6]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/7_July_2009 7]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/8_July_2009 8]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/9_July_2009 9]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/10_July_2009 10]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_July_2009&action=edit 13]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/14_July_2009 14]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/15_July_2009 15]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/16_July_2009 16]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_July_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_July_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_July_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_July_2009&action=edit 22]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/23_July_2009 23]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/24_July_2009 24]	[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/25_July_2009 25]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_July_2009&action=edit 26]
[http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/27_July_2009 27]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_July_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_July_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/30_July_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/31_July_2009&action=edit 31]

= Introductory Lab Sessions

Formal Lab Sessions (23rd July - 16th October 2009)

July
M	T	W	T	F	S	S
		[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/1_July_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/2_July_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/3_July_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/6_July_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/7_July_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/8_July_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/9_July_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/10_July_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/13_July_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/14_July_2009&action=edit 14]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/15_July_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/16_July_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/17_July_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/20_July_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/21_July_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/22_July_2009&action=edit 22]	[http://2009.igem.org/Team:Newcastle/Labwork/23_July_2009 23]	[http://2009.igem.org/Team:Newcastle/Labwork/24_July_2009 24]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/26_July_2009&action=edit 26]
[http://2009.igem.org/Team:Newcastle/Labwork/27_July_2009 27]	[http://2009.igem.org/Team:Newcastle/Labwork/28_July_2009 28]	[http://2009.igem.org/Team:Newcastle/Labwork/29_July_2009 29]	[http://2009.igem.org/Team:Newcastle/Labwork/30_July_2009 30]	[http://2009.igem.org/Team:Newcastle/Labwork/31_July_2009 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/1_August_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/3_August_2009&action=edit 3]	[http://2009.igem.org/Team:Newcastle/Labwork/4_August_2009 4]	[http://2009.igem.org/Team:Newcastle/Labwork/5_August_2009 5]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/6_August_2009&action=edit 6]	[http://2009.igem.org/Team:Newcastle/Labwork/7_August_2009 7]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/8_August_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/9_August_2009&action=edit 9]
[http://2009.igem.org/Team:Newcastle/Labwork/10_August_2009 10]	[http://2009.igem.org/Team:Newcastle/Labwork/11_August_2009 11]	[http://2009.igem.org/Team:Newcastle/Labwork/12_August_2009 12]	[http://2009.igem.org/Team:Newcastle/Labwork/13_August_2009 13]	[http://2009.igem.org/Team:Newcastle/Labwork/14_August_2009 14]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/15_August_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/16_August_2009&action=edit 16]
[http://2009.igem.org/Team:Newcastle/Labwork/17_August_2009 17]	[http://2009.igem.org/Team:Newcastle/Labwork/18_August_2009 18]	[http://2009.igem.org/Team:Newcastle/Labwork/19_August_2009 19]	[http://2009.igem.org/Team:Newcastle/Labwork/20_August_2009 20]	[http://2009.igem.org/Team:Newcastle/Labwork/21_August_2009 21]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/22_August_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/23_August_2009&action=edit 23]
[http://2009.igem.org/Team:Newcastle/Labwork/24_August_2009 24]	[http://2009.igem.org/Team:Newcastle/Labwork/25_August_2009 25]	[http://2009.igem.org/Team:Newcastle/Labwork/26_August_2009 26]	[http://2009.igem.org/Team:Newcastle/Labwork/27_August_2009 27]	[http://2009.igem.org/Team:Newcastle/Labwork/28_August_2009 28]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/29_August_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/31_August_2009&action=edit 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/Team:Newcastle/Labwork/1_September_2009 1]	[http://2009.igem.org/Team:Newcastle/Labwork/2_September_2009 2]	[http://2009.igem.org/Team:Newcastle/Labwork/3_September_2009 3]	[http://2009.igem.org/Team:Newcastle/Labwork/4_September_2009 4]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/5_September_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/6_September_2009&action=edit 6]
[http://2009.igem.org/Team:Newcastle/Labwork/7_September_2009 7]	[http://2009.igem.org/Team:Newcastle/Labwork/8_September_2009 8]	[http://2009.igem.org/Team:Newcastle/Labwork/9_September_2009 9]	[http://2009.igem.org/Team:Newcastle/Labwork/10_September_2009 10]	[http://2009.igem.org/Team:Newcastle/Labwork/11_September_2009 11]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/12_September_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/13_September_2009&action=edit 13]
[http://2009.igem.org/Team:Newcastle/Labwork/14_September_2009 14]	[http://2009.igem.org/Team:Newcastle/Labwork/15_September_2009 15]	[http://2009.igem.org/Team:Newcastle/Labwork/16_September_2009 16]	[http://2009.igem.org/Team:Newcastle/Labwork/17_September_2009 17]	[http://2009.igem.org/Team:Newcastle/Labwork/18_September_2009 18]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/19_September_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/20_September_2009&action=edit 20]
[http://2009.igem.org/Team:Newcastle/Labwork/21_September_2009 21]	[http://2009.igem.org/Team:Newcastle/Labwork/22_September_2009 22]	[http://2009.igem.org/Team:Newcastle/Labwork/23_September_2009 23]	[http://2009.igem.org/Team:Newcastle/Labwork/24_September_2009 24]	[http://2009.igem.org/Team:Newcastle/Labwork/25_September_2009 25]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/26_September_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/27_September_2009&action=edit 27]
[http://2009.igem.org/Team:Newcastle/Labwork/28_September_2009 28]	[http://2009.igem.org/Team:Newcastle/Labwork/29_September_2009 29]	[http://2009.igem.org/Team:Newcastle/Labwork/30_September_2009 30]

October
M	T	W	T	F	S	S
			[http://2009.igem.org/Team:Newcastle/Labwork/1_October_2009 1]	[http://2009.igem.org/Team:Newcastle/Labwork/2_October_2009 2]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/3_October_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/4_October_2009&action=edit 4]
[http://2009.igem.org/Team:Newcastle/Labwork/5_October_2009 5]	[http://2009.igem.org/Team:Newcastle/Labwork/6_October_2009 6]	[http://2009.igem.org/Team:Newcastle/Labwork/7_October_2009 7]	[http://2009.igem.org/Team:Newcastle/Labwork/8_October_2009 8]	[http://2009.igem.org/Team:Newcastle/Labwork/9_October_2009 9]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/10_October_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/11_October_2009&action=edit 11]
[http://2009.igem.org/Team:Newcastle/Labwork/12_October_2009 12]	[http://2009.igem.org/Team:Newcastle/Labwork/13_October_2009 13]	[http://2009.igem.org/Team:Newcastle/Labwork/14_October_2009 14]	[http://2009.igem.org/Team:Newcastle/Labwork/15_October_2009 15]	[http://2009.igem.org/Team:Newcastle/Labwork/16_October_2009 16]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/17_October_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/18_October_2009&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/19_October_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/20_October_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/21_October_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/22_October_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/23_October_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/24_October_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/26_October_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/27_October_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/28_October_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/29_October_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/30_October_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/31_October_2009&action=edit 31]

= Formal Lab Sessions

Protocols

Read this page to find out about the protocols used by Team:Newcastle in labs.

Lab rules

To ensure the team carried out the iGEM project safely, lab rules were seriously followed - to read them please click here

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

Newcastle iGEM Twitter
[http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
[http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]

@@ Line 3: / Line 3: @@
 {{:Team:Newcastle/Left}}
 __NOTOC__
-[[Image:Team-Newcastle iGem 2009 - Lab 03-07-09 no 1.JPG|thumb| Jane and Jess calculating serial dilutions]]
 =Lab Book=
+[[Image:Team Newcastle 2009 iGEM Animation22.gif|center]]
+<br>
 == Introductory Lab Sessions (30th June - 27th July)==
 To train up the members of our team who hadn't experienced lab work before, and to sharpen the skills of the team members who had, our advisers planned a period of introductory lab sessions. These experiments were based around growing cell cultures and making ''E.coli'' cells competent whilst making us aware of important lab principles such as handling of equipment, aseptic technique, lab etiquette, etc. '''Please see calendar below:'''
+<center>
 {|cellpadding="10"
 |{{#calendar: title=Team:Newcastle/IntroductoryLabwork |year=2009 | month=06 }}
 |{{#calendar: title=Team:Newcastle/IntroductoryLabwork |year=2009 | month=07 }}
 |}
-[[Image:Team Newcastle 2009 iGEM IntroductoryLabSessions.PNG|50px]] = Introductory Lab Sessions
+</center>
+[[Image:Team Newcastle 2009 iGEM IntroductoryLabSessions.PNG|30px]] = Introductory Lab Sessions
 <br>
-== Formal Lab Sessions ==
-{|cellpadding="10"
-|{{#calendar: title=Team:Newcastle/Labwork |year=2009 | month=07 }}
-|{{#calendar: title=Team:Newcastle/Labwork |year=2009 | month=08 }}
-|{{#calendar: title=Team:Newcastle/Labwork |year=2009 | month=09 }}
-|}
-[[Image:Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG|50px]] = Formal Lab Sessions
 <br>
-== Lab Rota==
-[[Image:Team Newcastle iGEM 2009 16-07-09 IMG 0071.JPG|thumb|200px|The labs have been a great learning experience]]
+== Formal Lab Sessions (23rd July - 16th October 2009) ==
-Read [[Team:Newcastle/Project/Labwork/Rota|this page]] to find out when members of Team:Newcastle are in labs.
+{{:Team:Newcastle/Project/Labwork/CalTemplate}}
+[[Image:Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG|40px]] = Formal Lab Sessions
+<br>
+<br>
 == Protocols ==
 Read [[Team:Newcastle/Project/Labwork/Protocols|this page]] to find out about the protocols used by Team:Newcastle in labs.
-== All our stocks in Dr.Phil Aldridge's lab ==
-Read [[Team:Newcastle/Project/Labwork/Stocks|this page]] to find out about the stocks
 ==Lab rules==