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Team:Newcastle/Labwork/24 August 2009
From 2009.igem.org
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=Formal Lab Session - 24th August 2009= | =Formal Lab Session - 24th August 2009= | ||
| - | ==<u>Stochastic Switch | + | [[Image:Team Newcastle 2009 iGEM 24-08-09 IMG 0518.JPG|350px|center]] |
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| + | =<font color="Orange"><u>Overview</u></font>= | ||
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| + | *[[#Stochastic Switch Team|Stochastic Switch Team]] '''- prepared overnight cultures from transformant ''E. coli'' cells containing BioBricks from last week, i.e. ''BBa_R0062, BBa_R0079, BBa_C0161, BBa_C0179'', and ''BBa_J44000''''' | ||
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| + | ==<u>Stochastic Switch Team</u>== | ||
[[Image:Team Newcastle 2009 iGEM 24-08-09 IMG 0519.JPG|200px|thumb|Jess preparing to innoculate 3ml of LB with some transformant cultures]] | [[Image:Team Newcastle 2009 iGEM 24-08-09 IMG 0519.JPG|200px|thumb|Jess preparing to innoculate 3ml of LB with some transformant cultures]] | ||
===Summary=== | ===Summary=== | ||
Today was dedicated to finishing our cloning strategies and primer designs, and so lab was called off. However the stochastic switch team had time to set up some overnight cultures to redo the minipreps and restriction digests for the biobricks from last week. These will be carried out tomorrow. We mixed the colonies in 3 ml of LB+Amp and left them overnight in shaking incubator. To prepare LB + Amp solution we used 5ml of LB + 250ul of Amp. 12 cultures were left overnight. | Today was dedicated to finishing our cloning strategies and primer designs, and so lab was called off. However the stochastic switch team had time to set up some overnight cultures to redo the minipreps and restriction digests for the biobricks from last week. These will be carried out tomorrow. We mixed the colonies in 3 ml of LB+Amp and left them overnight in shaking incubator. To prepare LB + Amp solution we used 5ml of LB + 250ul of Amp. 12 cultures were left overnight. | ||
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{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
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Latest revision as of 19:08, 20 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Formal Lab Session - 24th August 2009
Overview
- Stochastic Switch Team - prepared overnight cultures from transformant E. coli cells containing BioBricks from last week, i.e. BBa_R0062, BBa_R0079, BBa_C0161, BBa_C0179, and BBa_J44000
Stochastic Switch Team
Summary
Today was dedicated to finishing our cloning strategies and primer designs, and so lab was called off. However the stochastic switch team had time to set up some overnight cultures to redo the minipreps and restriction digests for the biobricks from last week. These will be carried out tomorrow. We mixed the colonies in 3 ml of LB+Amp and left them overnight in shaking incubator. To prepare LB + Amp solution we used 5ml of LB + 250ul of Amp. 12 cultures were left overnight.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
