Team:UNIPV-Pavia/Notebook/Week3Dec

From 2009.igem.org

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= Week from December 15th, to December 21st, 2008 =
= Week from December 15th, to December 21st, 2008 =
== <font class="dayw_style">December, 16th</font> ==
== <font class="dayw_style">December, 16th</font> ==
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===Trasformation of Ligations===
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Presentation of the project "Engineering E. coli to multiplex and demultiplex signals" by UNIPV-Pavia iGEM 2008 team and call for participants for iGEM 2009 team.
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===Trasformation of Ligations===
 
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A5: BBa_Q0040 with BBa_I732017 (RBS-TETR-PTET-RBS-LACZ-TT)<br>
 
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A6: BBa_J23100 with BBa_Q0040 (PCON-RBS-TETR-TT-PTET )
 
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* 1 ul A5 in Top10 Competent
 
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* 1 ul A6 in Top10 Competent
 
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Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.
 
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* Add 250 ul SOC in 50 ul A5+ Top10
 
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* Add 250 ul SOC in 50 ul A6+ Top10
 
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1 hr incubation at 37°C (falcons with shaking 220 rpm) <br>
 
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Infect:
 
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# X-GAL plate with 200 ul A5
 
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# X-GAL plate with 100 ul A5
 
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# Agar plate with 200 ul A6
 
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# X-GAL plate with 20 ul of BBa_E0240 from glycerol stock (negative control).
 
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Expected results:
 
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*Plates 1 and 2 should develop blue colonies
 
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*Plate 4 should remain colorless
 
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16 hr incubation at 37°C.
 

Revision as of 09:03, 20 June 2009

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December 2008
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March 2009
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April 2009
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May 2009
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June 2009
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July 2009
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September 2009
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November 2009
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Week from December 15th, to December 21st, 2008

December, 16th

Trasformation of Ligations

Presentation of the project "Engineering E. coli to multiplex and demultiplex signals" by UNIPV-Pavia iGEM 2008 team and call for participants for iGEM 2009 team.

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