Team:BIOTEC Dresden/Project v2
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The project is split into three parts | The project is split into three parts | ||
+ | '''[[Notebook_Recombinase|FLP Recombinase-based PoPS Measurement Device ]]''' | ||
- | + | Utilizing a new biobrick, a recombinase mechanism is used to... | |
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Instead of gene expression in cells, it's attempted in vitro in vesicles. | Instead of gene expression in cells, it's attempted in vitro in vesicles. | ||
+ | '''[[Notebook_SilverNano|Silver Nano-Particles ]]''' | ||
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+ | Here, it's attempted to create nanoparticles using a silver-binding peptide, as described in... | ||
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{{:Team:BIOTEC_Dresden/NewTemplateEnd}} | {{:Team:BIOTEC_Dresden/NewTemplateEnd}} |
Revision as of 16:06, 19 October 2009
Temporal and spatial control of protein synthesis by in vitro recombination inside picoliter reactors
Manufacturing functionalized proteins in vitro poses a challenge, as it requires coordinated molecular assemblies and multi-step reactions. In this project we aim to control, over time and space, the production of proteins tagged with a silver-binding peptide for in situ silver nanoparticle nucleation inside microdroplets generated by microfluidic devices.
Combining a transcription-translation system with protein coding genes and a recombination logic inside microdroplets provides spatial control. Moreover, in the microfluidic chamber we can pinpoint the beginning of synthesis, and easily track and isolate the droplets. Site-specific recombination generates a molecular timer for temporal control of protein synthesis.
Unlike transcriptional regulation, this method gives true all-or-none induction due to covalent modification of DNA by Flp recombinase. Determining the transfer curve of inter-FRT site distance versus average recombination time allows the onset of gene expression to be predicted. We then apply this Flp reporter system as a powerful PoPS measurement device.
The project is split into three parts
FLP Recombinase-based PoPS Measurement Device
Utilizing a new biobrick, a recombinase mechanism is used to...
Instead of gene expression in cells, it's attempted in vitro in vesicles.
Here, it's attempted to create nanoparticles using a silver-binding peptide, as described in...
.