Team:Todai-Tokyo/Protocols/Notebook Sample

From 2009.igem.org

(Difference between revisions)
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The yqiT gene was [[Team:Todai-Tokyo/Protocols/PCR|PCR]] amplified from pLacI-yqiT-dterm ([[Team:Todai-Tokyo/Protocols/Miniprep|miniprep]]ped on 9/7) using the following primers:
The yqiT gene was [[Team:Todai-Tokyo/Protocols/PCR|PCR]] amplified from pLacI-yqiT-dterm ([[Team:Todai-Tokyo/Protocols/Miniprep|miniprep]]ped on 9/7) using the following primers:
-
yqiT fwd: agcccgtgtagtactgtagagt
+
'''yqiT fwd''': agcccgtgtagtactgtagagtt <BR>
-
yqiT rev: agggatgtttgccagtcgtaattag
+
'''yqiT rev''': agggatgtttgccagtcgtaattag <BR>
-
Using [[Team:Todai-Tokyo/Protocols/PCR|PCR program 1]]
+
using [[Team:Todai-Tokyo/Protocols/PCR/programs|PCR program 1]] and [[Team:Todai-Tokyo/Protocols/PCR/Enzymes|Ex-taq]].
 +
 
 +
This PCR reaction attaches a Biobrick prefix and suffix to the gene.
<h2> Sequencing </h2>
<h2> Sequencing </h2>
 +
 +
<h2> Transformation </h2>
<h2> Transformation </h2>
<h2> Ligation </h2>
<h2> Ligation </h2>

Revision as of 02:43, 20 October 2009

UT.png
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Contents

Miniprep

The following were miniprepped:

  • pLacI-RFP-dterm (From Ligation on 9/4; 4 colonies)
  • RFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] from [http://partsregistry.org/assembly/libraries.cgi?id=19 2009 Spring Distribution]; 6 colonies)

PCR

The yqiT gene was PCR amplified from pLacI-yqiT-dterm (miniprepped on 9/7) using the following primers:

yqiT fwd: agcccgtgtagtactgtagagtt
yqiT rev: agggatgtttgccagtcgtaattag

using PCR program 1 and Ex-taq.

This PCR reaction attaches a Biobrick prefix and suffix to the gene.


Sequencing


Transformation

Ligation

Infusion

Gel Purification

RE Digest

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