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Team:Newcastle/Labwork/8 October 2009
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=Formal Lab Session - 8th October 2009= | =Formal Lab Session - 8th October 2009= | ||
| - | Today we did restriction digets again with pmutin4 with the new enzymes. We concluded that the enzymes we used may have | + | == Introduction == |
| - | been contaminated. We ordered normal new enzymes and used them instead. | + | *Today we did restriction digets again with pmutin4 with the new enzymes. We concluded that the enzymes we used may have been contaminated. We ordered normal new enzymes and used them instead. |
| + | *We also did PCR fragment digests (from the cleaned PCr product) with BamHI and HindIII | ||
| + | * We run the digestions on the gel and cut PCR fragment and pmutin4 from the gel to clean up. | ||
| - | pmutin4 digest with HindIII and BamHI | + | == Experiment procedure == |
| + | |||
| + | === pmutin4 digest with HindIII and BamHI === | ||
H2O 26ul | H2O 26ul | ||
10X Buffer E 5ul | 10X Buffer E 5ul | ||
| Line 17: | Line 21: | ||
BamHI 1ul | BamHI 1ul | ||
| - | pmutin4 control digest with HindIII | + | === pmutin4 control digest with HindIII === |
H2O 13.5 ul | H2O 13.5 ul | ||
10X Buffer E 1ul | 10X Buffer E 1ul | ||
| Line 24: | Line 28: | ||
HindIII 0.5ul | HindIII 0.5ul | ||
| - | pmutin4 control digest with BamHI | + | === pmutin4 control digest with BamHI === |
H2O 13.5 ul | H2O 13.5 ul | ||
10X Buffer E 1ul | 10X Buffer E 1ul | ||
| Line 31: | Line 35: | ||
BamHI 0.5ul | BamHI 0.5ul | ||
| - | pSB1AT3 control digest with HindIII and BamHI | + | === pSB1AT3 control digest with HindIII and BamHI === |
H2O 13 ul | H2O 13 ul | ||
10X Buffer E 1ul | 10X Buffer E 1ul | ||
| Line 39: | Line 43: | ||
BamHI 0.5ul | BamHI 0.5ul | ||
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{{:Team:Newcastle/Project/Labwork/CalTemplate}} | {{:Team:Newcastle/Project/Labwork/CalTemplate}} | ||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} | ||
Revision as of 01:56, 21 October 2009
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Contents |
Formal Lab Session - 8th October 2009
Introduction
- Today we did restriction digets again with pmutin4 with the new enzymes. We concluded that the enzymes we used may have been contaminated. We ordered normal new enzymes and used them instead.
- We also did PCR fragment digests (from the cleaned PCr product) with BamHI and HindIII
- We run the digestions on the gel and cut PCR fragment and pmutin4 from the gel to clean up.
Experiment procedure
pmutin4 digest with HindIII and BamHI
H2O 26ul 10X Buffer E 5ul BSA 0.5 ul (diluted) DNA 15ul HindIII 1ul BamHI 1ul
pmutin4 control digest with HindIII
H2O 13.5 ul 10X Buffer E 1ul BSA 0.5 ul (diluted) DNA 3ul HindIII 0.5ul
pmutin4 control digest with BamHI
H2O 13.5 ul 10X Buffer E 1ul BSA 0.5 ul (diluted) DNA 3ul BamHI 0.5ul
pSB1AT3 control digest with HindIII and BamHI
H2O 13 ul 10X Buffer E 1ul BSA 0.5 ul (diluted) DNA 3ul HindIII 0.5ul BamHI 0.5ul
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
