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Team:UNIPV-Pavia/Notebook/Week3Sep
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== <html><font class="dayw_style">September, 17th</font></html> == | == <html><font class="dayw_style">September, 17th</font></html> == | ||
''Experiment with Tecan F200'' | ''Experiment with Tecan F200'' | ||
| - | *<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/A1A2A7 TEST 17-09-09.pdf" target="_blank"></a></html> | + | *<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/A1A2A7 TEST 17-09-09.pdf" target="_blank">Download Protocol</a></html> |
===== pH sensor ===== | ===== pH sensor ===== | ||
Revision as of 14:47, 20 October 2009
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Week from September 14th, to September 20th, 2009
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September, 14th
September, 15th
September, 16th
September, 17th
Experiment with Tecan F200
pH sensor
- Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
- RBS33
- A2
- K116002 (from now on it will be indicated only as pNhaA)
- Overnight incubation of these three cultures at 37°C, 220 rpm.
September, 18th
pH sensor
1st experiment
- We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
- OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
- We diluted:
- RBS into:
- LBK + Amp pH 5,5
- LBK + Amp pH 6,6
- LBK + Amp pH 7,5
- LBK + Amp pH 8,5
- pNhaA into:
- LBK + Amp pH 5,5
- LBK + Amp pH 6,6
- LBK + Amp pH 7,5
- LBK + Amp pH 8,5
- A2 into:
- LBK + Amp pH 5,5
- LBK + Amp pH 6,6
- LBK + Amp pH 7,5
- LBK + Amp pH 8,5
- RBS into:
- We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
September, 19th
September, 20th
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