Team:PKU Beijing/Parts Characterization/BBa K228260
From 2009.igem.org
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<partinfo>BBa_K228260 short</partinfo> | <partinfo>BBa_K228260 short</partinfo> | ||
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- | |[[ | + | |[[Team:PKU_Beijing/Parts_Characterization/BBa_K228260| Part Main Page ]]||[[Team:PKU_Beijing/Parts_Characterization/BBa_K228260:Protocol| Protocol ]] |
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In order to make the AND gate inducible, the researchers chose two promoters that can be induced by specific small molecules. And inspired by the conception of modulation put forward by the authors, we have swapped the promoters, expecting to obtain a better AND gate. Because the core interactions between the amber mutated DNA and the suppressor tRNA are not influenced, the alternation of promoters would not change the basic AND logic behavior. In addition, we also swapped various RBSs of T7ptag to regulate the relative quantity of mutated T7 polymerase and supD tRNA and thus facilitated the screening of a better AND gate. | In order to make the AND gate inducible, the researchers chose two promoters that can be induced by specific small molecules. And inspired by the conception of modulation put forward by the authors, we have swapped the promoters, expecting to obtain a better AND gate. Because the core interactions between the amber mutated DNA and the suppressor tRNA are not influenced, the alternation of promoters would not change the basic AND logic behavior. In addition, we also swapped various RBSs of T7ptag to regulate the relative quantity of mutated T7 polymerase and supD tRNA and thus facilitated the screening of a better AND gate. | ||
- | To see more [[ | + | To see more [[Team:PKU_Beijing/Parts_Characterization/BBa_K228260:Detail|details]]... |
This gate is one of the best AND gates we obtained, of which T7ptagn gene is regulated by BBa_J44001 RBS. | This gate is one of the best AND gates we obtained, of which T7ptagn gene is regulated by BBa_J44001 RBS. | ||
- | In order to characterize the AND gate quantificationally, we cloned a reporter gene gfp downstream of the T7 promoter to measure the output fluorescence intensity. The input promoters are activated in different degrees by varying the concentration of the arabinose and salicylate inducers from 10^-7 to 10^-3. (For more detail, refer to our [[ | + | In order to characterize the AND gate quantificationally, we cloned a reporter gene gfp downstream of the T7 promoter to measure the output fluorescence intensity. The input promoters are activated in different degrees by varying the concentration of the arabinose and salicylate inducers from 10^-7 to 10^-3. (For more detail, refer to our [[Team:PKU_Beijing/Parts_Characterization/BBa_K228260:Protocol|protocol]]) |
==='''Result'''=== | ==='''Result'''=== |
Revision as of 08:28, 21 October 2009
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