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Virginia Commonwealth/29 June 2009
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==Monday 29 June 2009== | ==Monday 29 June 2009== | ||
| + | ===Afton & Maria=== | ||
| + | ===Results=== | ||
| + | #There were no results from last night, since the cells were grown up in the wrong E. Coli. | ||
| + | ===Tasks=== | ||
| + | #Last night we had grown up the backbone PSB3K3 in E. Coli Strain NEB10β, however since it contained P1010 we should have used E. Coli strain DB3.1. | ||
| + | ====Wetlab==== | ||
| + | #Today we replated cells containing PSB3K3 in E. Coli Strain DB3.1. | ||
| + | #The controls for the experiment are: | ||
| + | ##E. Coli Cells on LB (+) | ||
| + | ##E. Coli Cells on LB with KAN (-) | ||
| + | ##E. Coli Cells Zapped on LB (+) | ||
| + | [[User:MandM|MandM]] 19:41, 29 June 2009 (UTC) | ||
| + | ===Kevin & Adam=== | ||
| + | ===Clay & Craig=== | ||
| + | |||
| + | |||
| + | |||
| + | |||
===Results=== | ===Results=== | ||
*1 There was no growth on the plate from last nights transformations. The wrong strain of E.coli was used. It did not have the death gene resistance. | *1 There was no growth on the plate from last nights transformations. The wrong strain of E.coli was used. It did not have the death gene resistance. | ||
Revision as of 19:41, 29 June 2009
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Contents |
Monday 29 June 2009
Afton & Maria
Results
- There were no results from last night, since the cells were grown up in the wrong E. Coli.
Tasks
- Last night we had grown up the backbone PSB3K3 in E. Coli Strain NEB10β, however since it contained P1010 we should have used E. Coli strain DB3.1.
Wetlab
- Today we replated cells containing PSB3K3 in E. Coli Strain DB3.1.
- The controls for the experiment are:
- E. Coli Cells on LB (+)
- E. Coli Cells on LB with KAN (-)
- E. Coli Cells Zapped on LB (+)
MandM 19:41, 29 June 2009 (UTC)
Kevin & Adam
Clay & Craig
Results
- 1 There was no growth on the plate from last nights transformations. The wrong strain of E.coli was used. It did not have the death gene resistance.
- 2
Tasks
- 1 Redo last nights transformations.
- 2 Make new LB plates.
=Wetlab
- 1 Kevin made fresh LB+agar and Craig poured and labeled the plates.
- 2
