Revision as of 14:21, 21 October 2009

Meetings Calendar

Metal Sequester

Introduction

In order to take up and keep the cadmium from the soil efficiently, we needed to think about a way to cross-link the metal ions to intra-cellular proteins which would end up for the most of it in the spore. From our meeting with Prof. Nigel Robinson on the 18th of March 2009, it was evident that the best way of getting cadmium into the spore is to express a metallothionein, which would 'soak up' the cadmium, which in turn would be trapped within the protein in the spore. Using the smtA metallothionein from E.coli and the CotC coat protein as a fusion partner, the metallothionein could in theory be localised to the spore coat as it forms (under control of mother cell specific SigK).

Novelty in this sub-project

To increase the efficiency of our system in sequestering the heavy metal cadmium from the soil, we divised a plan to make sure that most of the metal that have been taken in our B.subtilis cell is then rendered bio-unavailable by incorporating it into smtA metallothionein. We engineered the smtA gene so that it is fused with the spore coat protein cotC and the reporter gene GFP. This fusion will ensure that most of the smtAxpressed will be attached to the spore coat and therefore the heavy metal ions that are incorporated into the smtA protein will be localised into the spore.

@@ Line 12: / Line 12: @@
 ==Novelty in this sub-project==
-To increase the efficiency of our system in sequestering the heavy metal cadmium from the soil, we divised a plan to make sure that most of the metal that have been taken in our ''B.subtilis'' cell is then rendered bio-unavailable by incorporating it into ''smtA'' mettaloprotein which is expressed on the intracellular portion of the the spore coat
+To increase the efficiency of our system in sequestering the heavy metal cadmium from the soil, we divised a plan to make sure that most of the metal that have been taken in our ''B.subtilis'' cell is then rendered bio-unavailable by incorporating it into ''smtA'' metallothionein.
+We engineered the ''smtA'' gene so that it is fused with the spore coat protein cotC and the reporter gene GFP. This fusion will ensure that most of the ''smtA''xpressed will be attached to the spore coat and therefore the heavy metal ions that are incorporated into the ''smtA'' protein will be localised into the spore.
 ==Modelling==

Team:Newcastle/Metals

From 2009.igem.org

Revision as of 14:21, 21 October 2009

Metal Sequester

Introduction

Novelty in this sub-project

Modelling

BioBrick constructs

Lab Work Strategies

Other Presentations and Diagrams