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Team:UNIPV-Pavia/Notebook/Week2Jul
From 2009.igem.org
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*We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture (37°C, 220 rpm). | *We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture (37°C, 220 rpm). | ||
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| + | ''Preparation of experiment with Tecan F200'' | ||
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| + | *We picked a colony of B0030 plate (stored at +4°C) and infected 5 ml of LB + Amp. | ||
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| + | *We also infected 5 ml of LB + Amp with 10 ul of A1 and J23100 glycerol stocks. | ||
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| + | *We incubated the inocula overnight at 37°C, 220 rpm. | ||
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Revision as of 11:25, 7 July 2009
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Week from July 6th, to July 12nd, 2009
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July, 6th
- Digestion for:
| R0011(E-X) | BOL1(E-S) |
- Gel run/cut and band purification.
- The purified DNA gave a good result at Nanodrop, but we decided to perform the entire work on BOL1 again because we were not sure that we had extracted a pure band.
- We stored R0011(E-X) DNA at -20°C.
- We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture (37°C, 220 rpm).
Preparation of experiment with Tecan F200
- We picked a colony of B0030 plate (stored at +4°C) and infected 5 ml of LB + Amp.
- We also infected 5 ml of LB + Amp with 10 ul of A1 and J23100 glycerol stocks.
- We incubated the inocula overnight at 37°C, 220 rpm.
July, 7th
July, 8th
July, 9th
July, 10th
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