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Team:BIOTEC Dresden/Methods SilverNano
From 2009.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
Protocol 2: | Protocol 2: | ||
| + | A method designed by Fromm and Wennemers [2] was used to form silver nanoparticles by using a chemical reducing agent, sodium ascorbate (NaAsc.) | ||
*120 ul peptide (8ug/ul) | *120 ul peptide (8ug/ul) | ||
| - | *14 ul | + | *14 ul NaAsc. at 2mM |
*1.4 ul AgNO3 at 20mM | *1.4 ul AgNO3 at 20mM | ||
*4.6 ul H2O | *4.6 ul H2O | ||
| + | |||
| + | == == | ||
| + | [1]<br> | ||
| + | [2]K.Belser, K.Fromm, H.Wennemers, ''Angew. Chem. Int. Ed.'', 48, 3661-3664, 2009 | ||
{{:Team:BIOTEC_Dresden/NewTemplateEnd}} | {{:Team:BIOTEC_Dresden/NewTemplateEnd}} | ||
Revision as of 01:13, 22 October 2009
Silver Nano Particles - Methods
A ---- ml aliquot of the silver binding peptide ( sequence -----) was obtained from.... Two different experiments were setup using the following protocols
Protocol 1:
- 120 ul peptide (8ug/ul)
- 14 ul HEPES buffer at pH 7.5
- 1.4 ul AgNO3 at 20mM
- 4.6 ul H2O
Protocol 2:
A method designed by Fromm and Wennemers [2] was used to form silver nanoparticles by using a chemical reducing agent, sodium ascorbate (NaAsc.)
- 120 ul peptide (8ug/ul)
- 14 ul NaAsc. at 2mM
- 1.4 ul AgNO3 at 20mM
- 4.6 ul H2O
[1]
[2]K.Belser, K.Fromm, H.Wennemers, Angew. Chem. Int. Ed., 48, 3661-3664, 2009
