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Team:BIOTEC Dresden/Notebook Vesicles
From 2009.igem.org
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Revision as of 03:34, 22 October 2009
Gene Expression in vesicles
Strategy
Vesicles are formed using a microfluidic system, allowing for small reaction volumes and a controllable environment. The system is used to create emulsions of material in aqueous phase in a lipid phase. Proteins and various buffers are eventually added to the aqueous phase to carry out transcription and translation of the protein of interest in the vesicles, in this case, FLP recombinase. The production of FLP can be checked by tagging the final protein with a fluorescent protein like GFP, which can be then viewed using fluorescent microscopy.
The protocols used to create the microfluidic chamber and express genes in it are given here.
Here are the results for the vesicle formation in a microfluidic chamber.
