Team:IBB Pune/project/project3
From 2009.igem.org
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Project 3 is concerned with developing a system which is naturally competent. This is to be done by introducing ''com'' genes taken from naturally competent strains of ''Acinetobacter'' and ''Streptococcus'' into a suitable vector imparting natural competence and readiness of the bacterium to acquire genes. Induction of competence will thus enable the bacteria to take up naked DNA from its surroundings which will greatly facilitate transformation experiments. | Project 3 is concerned with developing a system which is naturally competent. This is to be done by introducing ''com'' genes taken from naturally competent strains of ''Acinetobacter'' and ''Streptococcus'' into a suitable vector imparting natural competence and readiness of the bacterium to acquire genes. Induction of competence will thus enable the bacteria to take up naked DNA from its surroundings which will greatly facilitate transformation experiments. | ||
This has several applications. | This has several applications. | ||
+ | <html> | ||
+ | <style type="text/css"> | ||
+ | #nav, #nav ul { | ||
+ | position: relative; | ||
+ | margin: 0 auto; /* purpose: allow centering ul table */ | ||
+ | padding: 0; | ||
+ | display: table /* purpose: ul doesn't stretch width 100% */ | ||
+ | } | ||
+ | |||
+ | #nav li { | ||
+ | display: table-cell; /* purpose: li behaves like table-cell */ | ||
+ | position: relative; /* purpose: non-overlap li elements in ul */ | ||
+ | list-style: none; /* purpose: remove default html list-style */ | ||
+ | } | ||
+ | |||
+ | #nav li a { | ||
+ | display: block; /* purpose: non-overlap div on a */ | ||
+ | margin: 0 1px 0 0; /* purpose: spacing main menu items */ | ||
+ | padding: 4px 59px; | ||
+ | background-color: #336633; | ||
+ | color: #FFF; | ||
+ | text-align: right; | ||
+ | text-decoration: none; /* purpose: remove underline from a */ | ||
+ | font: bold 13px arial; | ||
+ | } | ||
+ | |||
+ | #nav li a:hover { | ||
+ | background-color: #00CC33; | ||
+ | color: black; | ||
+ | } | ||
+ | |||
+ | #nav div { | ||
+ | position: absolute; /* purpose: li of div doesn't spread out */ | ||
+ | display: none; | ||
+ | width: 10em; | ||
+ | opacity: 0.8; | ||
+ | filter: alpha(opacity=80); | ||
+ | border: 1px solid #28B095; | ||
+ | background: #EAEBD8; | ||
+ | } | ||
+ | |||
+ | #nav span a, #nav div a { | ||
+ | position: relative; | ||
+ | display: block; /* purpose: a's in div have same width */ | ||
+ | margin: 0; | ||
+ | padding: 5px 10px; | ||
+ | text-align: left; | ||
+ | font: 11px arial; | ||
+ | } | ||
+ | |||
+ | #nav span a:hover, #nav div a:hover { | ||
+ | background-color: #00CC33; | ||
+ | color: #000; | ||
+ | } | ||
+ | |||
+ | #nav span div { | ||
+ | position: relative; | ||
+ | margin: 0; | ||
+ | border: none; /* purpose: reset border to none */ | ||
+ | border-top: 1px solid #5970B2; /* purpose: add a seperator */ | ||
+ | border-bottom: 1px solid #5970B2; /* purpose: add a seperator */ | ||
+ | opacity: 1.0; /* purpose: opacity already 0.8 by #nav div */ | ||
+ | filter: alpha(opacity=100); /* purpose: opacity already 80 by #nav div */ | ||
+ | } | ||
+ | |||
+ | #nav span div a { | ||
+ | text-indent: 10px; | ||
+ | } | ||
+ | |||
+ | #nav span div span div a { | ||
+ | text-indent: 20px; | ||
+ | } | ||
+ | |||
+ | #nav .expand { | ||
+ | background-image: url('https://static.igem.org/mediawiki/2008/e/ef/Icon-expand.png'); | ||
+ | background-repeat: no-repeat; | ||
+ | background-position: 95% 50%; | ||
+ | } | ||
+ | |||
+ | #nav .collapse { | ||
+ | background-image: url('https://static.igem.org/mediawiki/2008/c/cd/Icon-collapse.png'); | ||
+ | background-repeat: no-repeat; | ||
+ | background-position: 95% 50%; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <script type="text/javascript" src="http://www.kuleuven.be/bioscenter/igem/js/jquery.js"></script> | ||
+ | |||
+ | <script type="text/javascript"> | ||
+ | function toggleElement(layer){ | ||
+ | var myLayer = document.getElementById(layer); | ||
+ | if(myLayer.style.display=="none"){ | ||
+ | myLayer.style.display="block"; | ||
+ | myLayer.backgroundPosition="top"; | ||
+ | } else { | ||
+ | myLayer.style.display="none"; | ||
+ | } | ||
+ | } | ||
+ | </script> | ||
+ | |||
+ | <script type="text/javascript"> | ||
+ | |||
+ | function ddmsie() { | ||
+ | $("#nav ul").css('display', 'inline-block'); | ||
+ | $("#nav li").css('display', 'inline'); | ||
+ | $("#nav a").css('display', 'inline-block'); | ||
+ | $("#nav a").hover(function () {$(this).css('background-color', '#252025')}, | ||
+ | function () {$(this).css('background-color', '#649cd7')}); | ||
+ | $("#nav div a").css('display', 'block'); | ||
+ | $("#nav div").css('left', '0'); | ||
+ | $("#nav div").css('top', '100%'); | ||
+ | $("#nav span div").css('top', '0'); | ||
+ | } | ||
+ | |||
+ | function ddmozilla() { | ||
+ | |||
+ | } | ||
+ | |||
+ | function ddnav() { | ||
+ | $("#nav li").hover( | ||
+ | function () { | ||
+ | $(this).find("div:first").css('display', 'inline');}, | ||
+ | function () { | ||
+ | $(this).find("div:first").css('display', 'none');} | ||
+ | ); | ||
+ | |||
+ | $("#nav span > a").toggle( | ||
+ | function () { | ||
+ | $(this).removeClass("#nav expand").addClass("#nav collapse"); | ||
+ | $(this).css('background-color', '#99AAFF'); | ||
+ | $(this).parent().find("div:first").css('display', 'block');}, | ||
+ | function () { | ||
+ | $(this).removeClass("#nav collapse").addClass("#nav expand"); | ||
+ | $(this).hover( | ||
+ | function () { | ||
+ | $(this).css('background-color', '#d4e2ef');}, | ||
+ | function () { | ||
+ | $(this).css('background-color', '#649cd7');} | ||
+ | ); | ||
+ | $(this).parent().find("div:first").css('display', 'none'); | ||
+ | } | ||
+ | ).addClass("#nav expand"); | ||
+ | } | ||
+ | |||
+ | $(function () { | ||
+ | if(jQuery.browser.msie) ddmsie(); | ||
+ | if(jQuery.browser.mozilla) ddmozilla(); | ||
+ | ddnav(); | ||
+ | }); | ||
+ | </script> | ||
+ | <div align="center" id="nav"> | ||
+ | <ul> | ||
+ | |||
+ | <li><a href="https://2009.igem.org/Team:IBB_Pune">home</a></li> | ||
+ | |||
+ | <li><a href="https://2009.igem.org/Team:IBB_Pune/Team">team</a> | ||
+ | <div> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Team">Meet the Team</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/sponsors">Sponsors</a> | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | <li><a href="https://2009.igem.org/Team:IBB_Pune/Project">project</a> | ||
+ | <div> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Project">Summary</a> | ||
+ | <span><a>Details</a> | ||
+ | <div> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/project/project1">project1</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/project/project2"> project2</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/project/project3">project3</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/project/systems together">master plan</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/project/Results">results</a> | ||
+ | </div> | ||
+ | </span> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Modeling">Modeling</a> | ||
+ | <span><a>Related</a> | ||
+ | <div> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Applications">Applications</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/history">history</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/reference lit">Reading</a> | ||
+ | </div> | ||
+ | </span> | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li><a href="https://2008.igem.org/Team:ESBS-Strasbourg/Project">misc</a> | ||
+ | <div> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Links">Links</a> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Protocols">Protocols</a> | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li><a href="https://2009.igem.org/Team:IBB_Pune/Parts">parts</a> | ||
+ | <div> | ||
+ | <a href="https://2009.igem.org/Team:IBB_Pune/Parts">Submitted Parts</a> | ||
+ | <a href="#">Sandbox</a> | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li><a href="https://2009.igem.org/Team:IBB_Pune/Notebook">notebook</a> | ||
+ | |||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | </html> |
Revision as of 20:03, 10 July 2009
Project 3 is concerned with developing a system which is naturally competent. This is to be done by introducing com genes taken from naturally competent strains of Acinetobacter and Streptococcus into a suitable vector imparting natural competence and readiness of the bacterium to acquire genes. Induction of competence will thus enable the bacteria to take up naked DNA from its surroundings which will greatly facilitate transformation experiments.
This has several applications.