Virginia Commonwealth/13 July 2009
From 2009.igem.org
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* Digestion of J23100 | * Digestion of J23100 | ||
* Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) | * Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) | ||
+ | * Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone) | ||
* Plated: | * Plated: | ||
** (+) Control: NEB 10 beta on LB | ** (+) Control: NEB 10 beta on LB |
Revision as of 21:05, 13 July 2009
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Contents |
Monday 13 July 2009
Results
Maria and Afton
- Digestion was successful on parts J23106 and J06702
Trentay 18:30, 13 July 2009 (UTC)
Tasks
Maria and Afton
- Make daily planner for experimentation
- Perform Electrophoresis test on Digested parts J23106 and J06702
- Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Transform ligated DNA into e. coli NEB 10 beta
Trentay 18:34, 13 July 2009 (UTC)
Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
- 2
Wetlab
Maria and Afton
- Gel Electrophoresis
- Digestion of J23100
- Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone)
- Plated:
- (+) Control: NEB 10 beta on LB
- (+) Control: NEB 10 beta on LB shocked
- (-) Control: NEB 10 beta on Cm
- NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
Trentay 20:26, 13 July 2009 (UTC)