Team:Sweden

From 2009.igem.org

(Difference between revisions)
(→What is iGEM?)
(→Our project)
Line 15: Line 15:
=Our project=
=Our project=
----
----
-
::'''The effect of yeast mutants on the cell cycle by marking the stages of the cell cycle with fluorescent proteins'''
+
::'''The linguistic cell: Sentence parsing yeast'''
-
::Our goal is to create S. cerevisiae cells which have each cell cycle phase tagged with different fluorescent proteins.Specific aims are considered in conducting this project:
+
::Our goal is to create an finite state automaton in the cell which parses sentences according to their parts-of-speech.
-
:::#Creating an affective signaling strategy, i.e. either linking the fluorescent protein to the cyclin or linking the protein to the same promoter as the cyclin
+
-
:::#Tagging three or four of the cell cycle phases to observe the dynamic behavior
+
-
:::#Modeling the behavior of mutants and evaluating the model
+
-
 
+
-
::The different phases of the cell cycle (G1, S, G2, M) are controlled by specific CDK – complexes. These complexes are activated via a protein called cyclin. Activated cyclin – CDK – complexes promote the accelerated production of more cyclins. At a certain point this positive feedback loop turns into a negative feedback loop, i.e. the cyclin production comes to a stop and the complexes are turned off a cyclin degradation via ubiquitin.
+
-
::In the budding yeast Saccharomyces cere-visiae there are 9 different cyclins responsi-ble for the different phases. The cell cycle and the corresponding cyclins have been studied thoroughly.
+
-
::The positive correlation between cyclin expression and cell cycle phase can be used to dynamically visualize the cell cycle. There-fore we want to tag one cyclin for three phases while the fourth is the default.
+
-
::Tagging the cyclins will be done with GFP proteins. These proteins form a visible wavelength cromophore on their own with-out the help of any chaperones. GFP has been first discovered in the jellyfish Aequo-rea victoria. The wildtype GFP is very stable and is not degraded by most proteases therefore a lot of variants with varying char-acteristics have been engineered. These proteins have in common that they can be linked to another protein usually without affecting its function.
+
-
::Having the cells successfully tagged the cell cycle can be evaluated in different conditions (e. g. NaCl) and different yeast mutants can be analyzed with respect to the influence of the knocked-out gene on the cell cycle.
+
=The purpose of the project=
=The purpose of the project=

Revision as of 14:57, 9 August 2009

Home Our Team Our Project Project Design Log Book Result Modeling Sponsors
Head.jpg


Contents

Our project

The linguistic cell: Sentence parsing yeast
Our goal is to create an finite state automaton in the cell which parses sentences according to their parts-of-speech.

The purpose of the project

  1. This project might help to improve the yeast cell model and since S. cerevisiae is a model organism for eukaryotes the results might be used for medical purposes.
  2. This also implies a more accurate deterministic approach for Systems Biology/Synthetic Biology.
  3. Being able to analyze the whole cell cycle dynamically can help in determining the effect of drugs, e.g. in cancer research where drugs often inhibit or influence the cell cycle.

Funding of the iGEM team Sweden

iGEM is a great place for multinational companies to advertise as well as being seen by science community all over the world. iGEM is becoming one of the big-gest scientific competition and it is a great place for your company to show off. This year is the first year which Sweden has its own team in the competition. The Sweden team is supported by both Chalmers Technical University and Göteborg University and our supervisor is from the Wallenberg laboratory at the Sahlgrenska academy.

Team: Sweden

  • Sven Nelander –Supervisor
Postdoctoral Research Fellow
Dept of Molecular and Clinical Medicine
Email: sven.nelander@wlab.gu.se
  • Anna Hallerbach
Student: Bioinformatics, Chalmers
  • Fahim Mahmudur Rahman
Student: Bioinformatics , Chalmers
  • Guo Jing
Student: Bioinformatics , Chalmers
  • Laleh Kazemzadeh
Student: Bioinformatics, Chalmers
  • Mohammad Tanvir Ahamad
Student: Bioinformatics, Chalmers
  • Mohammad Intakhar Ahmad
Student: System Biology, Goteborg
  • Naser Monsefi
Student: System Biology, Goteborg
  • Tabassum Farzana Jahan
Student: Bioinformatics, Chalmers
  • Mao Tiezheng
Student: Bioinformatics, Chalmers


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your teams namespace.



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Example logo.png
Your team picture
Team Example


Home The Team The Project Parts Submitted to the Registry Modeling Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.) -->

Retrieved from "http://2009.igem.org/Team:Sweden"