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Revision as of 14:36, 21 July 2009 (view source) Trentay (Talk | contribs) (→Tuesday 21 July 2009) ← Older edit		Revision as of 14:47, 21 July 2009 (view source) Trentay (Talk | contribs) (→Tuesday 21 July 2009) Newer edit →
Line 57:		Line 57:
	** R0040 w/ E0240		** R0040 w/ E0240
	** R0040 w/ J06702		** R0040 w/ J06702
-	* Dilute	+	* Dilute overnight cultures for flow cytometer tomorrow
		+	** 5mL was diluted with 1 mL of overnight culture
		+	* Organize notebook and running draft
		+	* Pick colonies from transformation of J23100 and J23106 with RET E0240
	[[User:Trentay|Trentay]] 14:26, 21 July 2009 (UTC)		[[User:Trentay|Trentay]] 14:26, 21 July 2009 (UTC)
	----		----

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Revision as of 14:47, 21 July 2009

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Contents 1 Tuesday 21 July 2009 1.1 Results 1.2 Tasks 1.2.1 Wetlab

Tuesday 21 July 2009

Results

• Overnight cultures were successful
• Plated bacteria that were cultured overnight are still not expressing GFP and RFP
  • a colony of bacteria expressed RFP (to the naked eye) on a E0240 plate (RFP reporter)

Trentay 14:26, 21 July 2009 (UTC)

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Tasks

• Make Cryogenic stocks of overnight cultures (2 vials were made of each one)
  • J23110 w/ E0240
  • J23110 w/ J06702
  • J23113 w/ E0240
  • J23113 w/ J06702
  • J23116 w/ E0240
  • J23116 w/ J06702
  • R0011 w/ E0240
  • R0011 w/ J06702
  • R0040 w/ E0240
  • R0040 w/ J06702
• Dilute overnight cultures for flow cytometer tomorrow
  • 5mL was diluted with 1 mL of overnight culture
• Organize notebook and running draft
• Pick colonies from transformation of J23100 and J23106 with RET E0240

Trentay 14:26, 21 July 2009 (UTC)

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