Virginia Commonwealth/21 July 2009
From 2009.igem.org
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* Make Cryogenic stocks of overnight cultures (2 vials were made of each one) | * Make Cryogenic stocks of overnight cultures (2 vials were made of each one) | ||
** J23110 w/ E0240 | ** J23110 w/ E0240 | ||
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====Wetlab==== | ====Wetlab==== | ||
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- | *2 | + | * 2 |
+ | [[User:Trentay|Trentay]] 14:52, 21 July 2009 (UTC) |
Revision as of 14:52, 21 July 2009
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Contents |
Tuesday 21 July 2009
Results
- Overnight cultures were successful
- Plated bacteria that were cultured overnight are still not expressing GFP and RFP
- a colony of bacteria expressed RFP (to the naked eye) on a E0240 plate (RFP reporter)
Trentay 14:26, 21 July 2009 (UTC)
Tasks
- Make Cryogenic stocks of overnight cultures (2 vials were made of each one)
- J23110 w/ E0240
- J23110 w/ J06702
- J23113 w/ E0240
- J23113 w/ J06702
- J23116 w/ E0240
- J23116 w/ J06702
- R0011 w/ E0240
- R0011 w/ J06702
- R0040 w/ E0240
- R0040 w/ J06702
- Dilute overnight cultures for flow cytometer tomorrow
- 5mL was diluted with 1 mL of overnight culture
- Organize notebook and running draft
- Pick colonies from transformation of J23100 and J23106 with RET E0240
Trentay 14:26, 21 July 2009 (UTC)
Wetlab
- Cryogenic stocks were made
- 2
Trentay 14:52, 21 July 2009 (UTC)