Team:Newcastle/Labwork/27 July 2009

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(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the...)
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We checked the agar plates containing colonies of plasmids with five different biobricks.
We checked the agar plates containing colonies of plasmids with five different biobricks.
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Plate 1: Bba_C0056
+
{| class="wikitable" border="1"
-
Plate 2: Bba_B1002
+
|-
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Plate 3: Bba_
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| Plate
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Plate 4: Bba_C0076
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| BioBrick
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Plate 5: Bba_R0077
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|-
 +
| Plate 1
 +
| Bba_C0056
 +
|-
 +
| Plate 2
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| Bba_B1002
 +
|-
 +
| Plate 3
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| Bba_??
 +
|-
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| Plate 4
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| Bba_C0076
 +
|-
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| Plate 5
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| Bba_R0077
 +
|-
 +
|}
Expcept from plate 3 we had colonies formed in all plates.
Expcept from plate 3 we had colonies formed in all plates.

Revision as of 14:52, 27 July 2009

Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.

We checked the agar plates containing colonies of plasmids with five different biobricks.

Plate BioBrick
Plate 1 Bba_C0056
Plate 2 Bba_B1002
Plate 3 Bba_??
Plate 4 Bba_C0076
Plate 5 Bba_R0077

Expcept from plate 3 we had colonies formed in all plates.

we prepared 12 tubes for 4 different biobricks. Colonies were picked and mixed in a tube containing 5ml of LB. Finally we left them at shaking incubator overnight. We labelled the tubes as below: 1A: 1B: 1C: We then prepared the plates for the plasmids.




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