Virginia Commonwealth/27 July 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 16:57, 28 July 2009

May
M	T	W	T	F	S	S
				[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14]	[http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23]	[http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]

June
M	T	W	T	F	S	S
[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]

July
M	T	W	T	F	S	S
		[http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30]	[http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15]	[http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]

October
M	T	W	T	F	S	S
			[http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Monday 27 July 2009

Results

Kevin & Adam

Results from Friday's PCR product
##	A260	Volume (uL)	Concentration (ug/uL)	Amount (ug)
Design 1 0.0	.017	40.1	.034	1.36
Design 1 0.5	.018	40.1	.036	1.44
Design 1 1.0	.014	40.0	.028	1.12
Design 1 1.5	.012	40.4	.024	.97
Design 2	.047	39.4	.094	3.7
Design 3	.028	41.0	.056	2.29
Design 4	.039	48.0	.078	3.74
Design 5	.032	36.0	.064	2.30
Design 6	.030	39.7	.060	2.38
Design 7	.029	43.0	.058	2.49
Design 8	.022	39.2	.044	1.72
Design 9	.032	41.9	.064	2.68

For Digestion and Ligation of Promoters
##	A260	Volume (uL)	Concentration (ug/uL)	Amount (ug)
pSB4C5	.086	19.5	.172	3.18
J06702	.023	125.0	.040	5.75
BBa_J23101	.052	35.2	.104	3.66

Maria and Afton

Nothing is glowing except one colony, J23110 w/ 702

Trentay 16:57, 28 July 2009 (UTC)

Tasks

Kevin & Adam

Discuss and continue to study design principles for T7 and various other inducible/repressible promoters.

Maria and Afton

Regrow, Miniprep, Take spectrophotometry measurements, Digest, Ligate, Transform ligated parts:
- pSB4C5, J23110, J06702,
Troubleshoot and Repeat this process until RFP is expressed
Make overnight cultures of parts pSB4C5, J23110, J06702, J23110 w/ 702

Trentay 16:57, 28 July 2009 (UTC)

Wetlab

Kevin & Adam

Digest and Ligate synthetic promoter designs 1-9.

Maria and Afton

Overnight cultures were made (5mL of selective broth with 100 microliters of DNA)
- Samples taken from cryogenic stocks
  - J23110 (AMP)
  - J06702 (AMP)
  - pSB4C5 (Cm)
- Samples taken from plates
  - J23110 w/ 702

Trentay 16:57, 28 July 2009 (UTC)

@@ Line 82: / Line 82: @@
 |}
+''Maria and Afton''
+* Nothing is glowing except one colony, J23110 w/ 702
+[[User:Trentay|Trentay]] 16:57, 28 July 2009 (UTC)
 ----
 ===Tasks===
-----
 ''Kevin & Adam''
 *Discuss and continue to study design principles for T7 and various other inducible/repressible promoters.
+''Maria and Afton''
+* Regrow, Miniprep, Take spectrophotometry measurements, Digest, Ligate, Transform ligated parts:
+** pSB4C5, J23110, J06702,
+* Troubleshoot and Repeat this process until RFP is expressed
+* Make overnight cultures of parts pSB4C5, J23110, J06702, J23110 w/ 702
+[[User:Trentay|Trentay]] 16:57, 28 July 2009 (UTC)
+----
 ====Wetlab====
 ''Kevin & Adam''
 *Digest and Ligate synthetic promoter designs 1-9.
+''Maria and Afton''
+* Overnight cultures were made (5mL of selective broth with 100 microliters of DNA)
+** Samples taken from cryogenic stocks
+*** J23110 (AMP)
+*** J06702 (AMP)
+*** pSB4C5 (Cm)
+** Samples taken from plates
+*** J23110 w/ 702
+[[User:Trentay|Trentay]] 16:57, 28 July 2009 (UTC)

Virginia Commonwealth/27 July 2009

From 2009.igem.org

Latest revision as of 16:57, 28 July 2009

Contents

Monday 27 July 2009

Results

Tasks

Wetlab